EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.14 | detection of two adjacent genes (PF1233 and PF1234) homologous to chitinase genes of Thermococcus kodakarensis in pyrococcus furiosus. In the cultured medium of Pyrococcus furiosus no chitinase is detected. On analysis of the structural gene of Pyrococcus furiosus, it appears that one nucleotide insertion in PF1234 causes a frame shift and separates a gene. By deletion of one nucleotide in PF1234, the best match is achieved between chitinases of Thermococcus kodakarensis and Pyrococcus furiosus. An artificial recombinant chitinase is constructed, exhibiting hydrolytic activity towards colloidal and crystalline chitins at high temperatures. Expression in Escherichia coli | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.14 | Pyrococcus furiosus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.14 | recombinant enzyme | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.14 | chitin + H2O | recombinant enzyme hydrolyzes colloidal and crystalline chitins | Pyrococcus furiosus | ? | - |
? | |
3.2.1.14 | additional information | chitinase is not important for the growth of Pyrococcus furiosus | Pyrococcus furiosus | ? | - |
? |