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Literature summary extracted from

  • Genda, T.; Watabe, S.; Ozaki, H.
    Purification and characterization of fumarase from Corynebacterium glutamicum (2006), Biosci. Biotechnol. Biochem., 70, 1102-1109.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.2 dithiothreitol partially restores from urea and alkaline inactivation Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 (R)-malate mixed-type inhibition Corynebacterium glutamicum
4.2.1.2 ATP competitive or mixed-type inhibition Corynebacterium glutamicum
4.2.1.2 D-Tartrate competitive Corynebacterium glutamicum
4.2.1.2 meso-tartrate competitive Corynebacterium glutamicum
4.2.1.2 additional information no inactivation with L-tartrate, citrate, succinate, glycine, maleate, 1 mM iodoacetate, 1 mM iodoacetamide, 1 mM N-ethylmaleimide or 0.5 mM PCMB Corynebacterium glutamicum
4.2.1.2 pyromellitate competitive Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.38
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 0.67
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 0.81
-
 fumarate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 1.4
-
 (S)-malate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 1.8
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 1.8
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 3.1
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 3.8
-
 fumarate phosphate buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 4.2
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 5
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 6.3
-
 (S)-malate phosphate buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 13
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.2 Mg2+ 2 mM, slight stimulation Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 50000
-
 4 * 50000, SDS-PAGE Corynebacterium glutamicum
4.2.1.2 200000
-
 gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Corynebacterium glutamicum Q8NRN8 ATCC 14067
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 315
-
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Corynebacterium glutamicum fumarate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.2 tetramer 4 * 50000, SDS-PAGE Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 21
-
 pH 7.6, stable Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.2 34
-
 (S)-malate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 48
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 110
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 200
-
 (S)-malate phosphate buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 290
-
 (S)-malate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 310
-
 fumarate MES-NaOH buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 430
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 460
-
 (S)-malate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 510
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 650
-
 fumarate MES-NaOH buffer, pH 7.0 Corynebacterium glutamicum
4.2.1.2 720
-
 fumarate phosphate buffer, pH 6.0 Corynebacterium glutamicum
4.2.1.2 730
-
 fumarate phosphate buffer, pH 7.0 Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 6.5
-
 phosphate buffer, substrate: fumarate Corynebacterium glutamicum
4.2.1.2 7.5
-
 Tris-HCl buffer, substrate: fumarate Corynebacterium glutamicum
4.2.1.2 8.3
-
 phosphate buffer, substrate: malate Corynebacterium glutamicum
4.2.1.2 8.5
-
 Tris-HCl buffer, substrate: malate Corynebacterium glutamicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.2 7
-
 stable Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.2 0.11
-
 meso-tartrate
-
 Corynebacterium glutamicum
4.2.1.2 1.3
-
 pyromellitate
-
 Corynebacterium glutamicum
4.2.1.2 2.5
-
 (R)-malate
-
 Corynebacterium glutamicum
4.2.1.2 16
-
 D-Tartrate
-
 Corynebacterium glutamicum