BRENDA - Enzyme Database

Structure-activity analysis of base and enzyme-catalyzed 4-hydroxybenzoyl coenzyme A hydrolysis

Song, F.; Zhuang, Z.; Dunaway-Mariano, D.; Bioorg. Chem. 35, 1-10 (2007)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.006
-
4-hydroxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
5
20
4-fluorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
56
-
4-methoxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
230
-
4-methylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
300
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
510
-
benzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
550
-
4-chlorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.23
Pseudomonas sp.
P56653
CBS3, recombinantly expressed in Escherichia coli
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.23
4-Chlorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Chlorobenzoate + CoA
-
-
-
?
3.1.2.23
4-fluorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-fluorobenzoate + CoA
-
-
-
?
3.1.2.23
4-Hydroxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Hydroxybenzoate + CoA
-
-
-
?
3.1.2.23
4-methoxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methoxybenzoate + CoA
-
-
-
?
3.1.2.23
4-methylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methylbenzoate + CoA
-
-
-
?
3.1.2.23
4-trifluoromethylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-trifluoromethylbenzoate + CoA
-
-
-
?
3.1.2.23
Benzoyl-CoA + H2O
-
678580
Pseudomonas sp.
Benzoate + CoA
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.038
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
0.047
-
4-methylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
0.27
-
4-methoxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
1
-
benzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
1.1
-
4-chlorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
9.7
-
4-fluorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
18.3
-
4-hydroxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.006
-
4-hydroxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
5
20
4-fluorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
56
-
4-methoxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
230
-
4-methylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
300
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
510
-
benzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
550
-
4-chlorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.23
4-Chlorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Chlorobenzoate + CoA
-
-
-
?
3.1.2.23
4-fluorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-fluorobenzoate + CoA
-
-
-
?
3.1.2.23
4-Hydroxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Hydroxybenzoate + CoA
-
-
-
?
3.1.2.23
4-methoxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methoxybenzoate + CoA
-
-
-
?
3.1.2.23
4-methylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methylbenzoate + CoA
-
-
-
?
3.1.2.23
4-trifluoromethylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-trifluoromethylbenzoate + CoA
-
-
-
?
3.1.2.23
Benzoyl-CoA + H2O
-
678580
Pseudomonas sp.
Benzoate + CoA
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.2.23
0.038
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
0.047
-
4-methylbenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
0.27
-
4-methoxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
1
-
benzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
1.1
-
4-chlorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
9.7
-
4-fluorobenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.
3.1.2.23
18.3
-
4-hydroxybenzoyl-CoA
pH 7.5, 25C
Pseudomonas sp.