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Literature summary extracted from

  • Makde, R.D.; Dikshit, K.; Kumar, V.
    Protein engineering of class-A non-specific acid phosphatase (PhoN) of Salmonella typhimurium: modulation of the pH-activity profile (2006), Biomol. Eng., 23, 247-251.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.2 I78A mutant displays significantly higher (as high as 160%) enzymatic efficiency over a broad pH range of 3.0-9.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 I78D mutant possesses nearly twice the specific activity at its optimum pH of 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 I78H mutant exhibits an alkaline shift in the pH-optimum by 1.5 pH points relative to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.07
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.08
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.08
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.1
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.1
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.1
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.11
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.12
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.14
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.25
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.32
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.47
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.55
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 1.8
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Salmonella enterica subsp. enterica serovar Typhimurium Q71EB8
-
-
3.1.3.2 Salmonella enterica subsp. enterica serovar Typhimurium MD6001 Q71EB8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 Q-Sepharose column chromatography, SP-Sepharose column chromatography, and phenyl-Sepharose column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium p-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium MD6001 p-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 bNSAP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 class-A bacterial non-specific acid phosphatases
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 PhoN
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 0.41
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 0.99
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 2 8 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 3.5
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 3.8
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 5.9
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 8.1
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 11.83
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 12.1
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 16.1
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 16.9
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 18.24
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 19.7
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 3.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 19.95
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 20.84
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 21.53
-
 p-nitrophenyl phosphate mutant enzyme I78H, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 22.25
-
 p-nitrophenyl phosphate wild type enzyme, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 23.32
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 26.42
-
 p-nitrophenyl phosphate mutant enzyme I78A, in 100 mM sodium acetate buffer, at 37°C and pH 5.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 32.2
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 8.5 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 41.6
-
 p-nitrophenyl phosphate mutant enzyme I78D, in 100 mM sodium acetate buffer, at 37°C and pH 7.0 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5.5
-
 wild type enzyme and mutant enzyme I78A Salmonella enterica subsp. enterica serovar Typhimurium
3.1.3.2 7
-
 mutant enzymes I78D and I78H Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 3 9
-
 Salmonella enterica subsp. enterica serovar Typhimurium