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Literature summary extracted from

  • Yi, Y.J.; Manandhar, G.; Sutovsky, M.; Li, R.; Jonakova, V.; Oko, R.; Park, C.S.; Prather, R.S.; Sutovsky, P.
    Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization (2007), Biol. Reprod., 77, 780-793.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.19.12 medicine sperm acrosomal UCHs are involved in sperm-zona pellucida interactions and antipolyspermy defense. Modulation of spermal UCH activity may facilitate the management of polyspermy during in vitro fertilization and provide insights into male fertility. Recombinant UCHs reduce polyspermy and proportionally increase the rate of monospermic fertilization during in vitro fertilization. Oocyte-secreted UCHs are not necessary for antipolyspermy defense during in vitro fertilization Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.12 Antibody anti-UCH antibodies increase the rate of polyspermy during in vitro fertilization by reducing UCH enzymatic activity Sus scrofa
3.4.19.12 Ubiquitin aldehyde specific nonpermeating UCH inhibitor, signficantly increases rates of sperm-zona pellucida penetration and polyspermy during in vitro fertilization by reducing UCH enzymatic activity in motile boar spermatozoa. Ipase-T and ubiquitin aldehyde cancel each other out when added concomitantly to the fertilization medium Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.19.12 cytoplasm UCHL3 diffusely distributed in the zygotic cytoplasm after fertilization Sus scrofa 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.12 24000
-
 UCHL1 or UCHL3, Western blot analysis, immunocytochemistry Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.12 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.12 oocyte UCHL1 present in the cortex, UCHL3 is primarly associated with the meiotic spindle of metaphase II ova Sus scrofa
-
3.4.19.12 Sertoli cell UCHL3 Sus scrofa
-
3.4.19.12 sperm UCHL3 is localized to the acrosomal surface. UCHL1 is absent from the sperm surface Sus scrofa
-
3.4.19.12 spermatid UCHL3 mainly localized to the round spermatids (acrosomal cap) and elongating spermatids (caudal manchette and acrosome) Sus scrofa
-
3.4.19.12 spermatogonium UCHL1 Sus scrofa
-
3.4.19.12 testis UCHL1 and UCHL3 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.12 additional information UCHL3 interacts with peripheral acrosomal plasma membrane proteins Sus scrofa ?
-
 ?
3.4.19.12 ubiquitin-7-amido-4-methylcoumarin + H2O
-
 Sus scrofa ubiquitin + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.19.12 PGP9.5
-
 Sus scrofa
3.4.19.12 ubiquitin C-terminal hydrolase
-
 Sus scrofa
3.4.19.12 UCHL1
-
 Sus scrofa
3.4.19.12 Uchl3
-
 Sus scrofa