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Literature summary extracted from
- Kinetic properties of cathepsin D and BACE 1 indicate the need to search for additional beta-secretase candidate(s) (2008), Biol. Chem., 389, 313-320.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.23.5 | medicine | beta-secretase activity in brain is mainly due to cathepsin D. As cathepsin D shows poor activity towards the wild-type beta-site of the amyloid precursor protein it is necessary to continue the search for additional beta-secretase candidates | Homo sapiens |
| 3.4.23.5 | medicine | beta-secretase activity in brain is mainly due to cathepsin D. As cathepsin D shows poor activity towards the wild-type beta-site of the amyloid precursor protein it is necessary to continue the search for additional beta-secretase candidates | Sus scrofa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.5 | pepstatin A | strongly inhibits the cleavage of wild-tpye and mutated Swedish family peptide substrate bearing two mutated residues in the beta-site of the amyloid precursor protein | Homo sapiens |  |
| 3.4.23.5 | pepstatin A | strongly inhibits the cleavage of wild-tpye and mutated Swedish family peptide substrate bearing two mutated residues in the beta-site of the amyloid precursor protein | Sus scrofa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.5 | 0.62 | - |
EEISEVNLDAEFRG | - |
Sus scrofa | |
| 3.4.23.5 | 1.1 | - |
EEISEVNLDAEFRG | - |
Homo sapiens | |
| 3.4.23.5 | 1.45 | - |
SEVNLDAEFR | - |
Sus scrofa | |
| 3.4.23.5 | 1.67 | - |
SEVNLDAEFR | - |
Homo sapiens | |
| 3.4.23.46 | additional information | - |
additional information | ratio kcat/Km for substrates SEVKMDAEFR and EEISEVKMDAEFRG is 53.3 per M and s and 95 per M and s, respectively | Homo sapiens | |
| 3.4.23.46 | 1.71 | - |
EEISEVNLDAEFRG | in 44 mM acetic acid/6 mM Na-acetate pH 3.8, 10 mM beta-mercaptoethanol, at 37°C | Homo sapiens | |
| 3.4.23.46 | 1.71 | - |
EEISEVNLDAEFRG | pH 3.8, 37°C | Homo sapiens | |
| 3.4.23.46 | 4.07 | - |
SEVNLDAEFR | in 44 mM acetic acid/6 mM Na-acetate pH 3.8, 10 mM beta-mercaptoethanol, at 37°C | Homo sapiens | |
| 3.4.23.46 | 4.07 | - |
SEVNLDAEFR | pH 3.8, 37°C | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.46 | 70000 | - |
SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.5 | Homo sapiens | - |
- |
- |
| 3.4.23.5 | Sus scrofa | - |
- |
- |
| 3.4.23.46 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.5 | purified from a bovine brain homogenate | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.5 | brain | - |
Sus scrofa | - |
| 3.4.23.5 | brain | by Western blot analysis it is shown that cathepsin is approximately 280fold more abundant in human brain than BACE-1 | Homo sapiens | - |
| 3.4.23.46 | brain | - |
Homo sapiens | - |
| 3.4.23.46 | brain | both BACE1 and human cathepsin D show poor activity towards the wild-type amyloid beta-site sequence | Homo sapiens | - |
| 3.4.23.46 | cerebral cortex | - |
Homo sapiens | - |
| 3.4.23.46 | hippocampus | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.5 | EEISEVKMDAEFRG + H2O | beta-secretase activity assay: wild-type peptide substrate (14-mer) of the amyloid precursor protein. Cathepsin D shows only poor activity towards the wild-type beta-site sequence | Homo sapiens | EEISEVKM + DAEFRG | - |
? | |
| 3.4.23.5 | EEISEVKMDAEFRG + H2O | beta-secretase activity assay: wild-type peptide substrate (14-mer) of the amyloid precursor protein. Cathepsin D shows only poor activity towards the wild-type beta-site sequence | Sus scrofa | EEISEVKM + DAEFRG | - |
? | |
| 3.4.23.5 | EEISEVNLDAEFRG + H2O | beta-secretase activity assay: mutated Swedish family (SW) peptide subtrate (14-mer) with the two mutated residues flanking the beta-site of the amyloid precursor protein | Homo sapiens | EEISEVNL + DAEFRG | - |
? | |
| 3.4.23.5 | EEISEVNLDAEFRG + H2O | beta-secretase activity assay: mutated Swedish family (SW) peptide subtrate (14-mer) with the two mutated residues flanking the beta-site of the amyloid precursor protein | Sus scrofa | EEISEVNL + DAEFRG | - |
? | |
| 3.4.23.5 | SEVKMDAEFR + H2O | beta-secretase activity assay: wild-type peptide substrate (10-mer) of the beta-site of the amyloid precursor protein. Cathepsin D shows only poor activity towards the wild-type beta-site sequence | Homo sapiens | SEVKM + DAEFR | - |
? | |
| 3.4.23.5 | SEVKMDAEFR + H2O | beta-secretase activity assay: wild-type peptide substrate (10-mer) of the beta-site of the amyloid precursor protein. Cathepsin D shows only poor activity towards the wild-type beta-site sequence | Sus scrofa | SEVKM + DAEFR | - |
? | |
| 3.4.23.5 | SEVNLDAEFR + H2O | beta-secretase activity assay: mutated Swedish family (SW) peptide subtrate (10-mer) with the two mutated residues flanking the beta-site of the amyloid precursor protein | Homo sapiens | SEVNL + DAEFR | - |
? | |
| 3.4.23.5 | SEVNLDAEFR + H2O | beta-secretase activity assay: mutated Swedish family (SW) peptide subtrate (10-mer) with the two mutated residues flanking the beta-site of the amyloid precursor protein | Sus scrofa | SEVNL + DAEFR | - |
? | |
| 3.4.23.46 | beta-amyloid precursor protein APP + H2O | - |
Homo sapiens | beta-amyloid protein + beta-amyloid precursor protein pre-peptide | - |
? | |
| 3.4.23.46 | EEISEVKMDAEFRG + H2O | - |
Homo sapiens | EEISEVKM + DAEFRG | - |
? | |
| 3.4.23.46 | EEISEVNLDAEFRG + H2O | - |
Homo sapiens | EEISEVNL + DAEFRG | - |
? | |
| 3.4.23.46 | SEVKMDAEFR + H2O | - |
Homo sapiens | SEVKM + DAEFR | - |
? | |
| 3.4.23.46 | SEVNLDAEFR + H2O | - |
Homo sapiens | SEVNL + DAEFR | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.5 | cathepsin D | - |
Homo sapiens |
| 3.4.23.5 | cathepsin D | - |
Sus scrofa |
| 3.4.23.46 | BACE1 | - |
Homo sapiens |
| 3.4.23.46 | beta-secretase | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.5 | 1.5 | - |
EEISEVNLDAEFRG | - |
Sus scrofa | |
| 3.4.23.5 | 8.8 | - |
SEVNLDAEFR | - |
Homo sapiens | |
| 3.4.23.5 | 29 | - |
SEVNLDAEFR | - |
Sus scrofa | |
| 3.4.23.5 | 31.4 | - |
EEISEVNLDAEFRG | - |
Homo sapiens | |
| 3.4.23.5 | 56.1 | - |
EEISEVNLDAEFRG | - |
Sus scrofa | |
| 3.4.23.46 | 10.7 | - |
SEVNLDAEFR | in 44 mM acetic acid/6 mM Na-acetate pH 3.8, 10 mM beta-mercaptoethanol, at 37°C | Homo sapiens | |
| 3.4.23.46 | 10.7 | - |
SEVNLDAEFR | pH 3.8, 37°C | Homo sapiens | |
| 3.4.23.46 | 17.6 | - |
EEISEVNLDAEFRG | in 44 mM acetic acid/6 mM Na-acetate pH 3.8, 10 mM beta-mercaptoethanol, at 37°C | Homo sapiens | |
| 3.4.23.46 | 17.6 | - |
EEISEVNLDAEFRG | pH 3.8, 37°C | Homo sapiens | |
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