EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.B43 | expressed in NS0 myeloma cell line | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.B43 | 3,4-dichloroisocoumarin | strong inhibitor at 0.25 mM | Homo sapiens | |
3.4.21.B43 | Aprotinin | weak inhibition at 0.02 mM | Homo sapiens | |
3.4.21.B43 | benzamidine | strong inhibitor at 5 mM | Homo sapiens | |
3.4.21.B43 | additional information | no inhibition is observed for soybean trypsin inhibitor at concentrations up to 500 nM, alpha1-antichymotrypsin, kallistatin, plasminogen activator inhibitor-1, and alpha2-macroglobulin | Homo sapiens | |
3.4.21.B43 | NaCl | activity is reduced by approximately 30% in the presence of 0.8 M NaCl | Homo sapiens | |
3.4.21.B43 | Zn2+ | 10 mM Zn2+ is sufficient to inhibit 50% of the KLK12 enzyme activity, more than 95% suppression can be achieved by 0.2 mM Zn2+ | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B43 | 0.0294 | - |
D-Val-Leu-Lys-thiobenzyl ester | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 0.0686 | - |
tert-butyloxycarbonyl-QAR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 0.2 | - |
tert-butyloxycarbonyl-VPR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 0.35 | - |
tert-butyloxycarbonyl-DPR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 0.565 | - |
tert-butyloxycarbonyl-FSR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.B43 | Ca2+ | compared to the absence of calcium, a 2fold, 2.5fold, and 3fold increase in activity could be obtained by adding Ca2+ concentration of 0.1, 1, and 10 mM, respectively, however, it is not increased further by higher concentrations of Ca2+ | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.B43 | 38000 | - |
SDS-PAGE | Homo sapiens |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.4.21.B43 | Brij-35 | adding 10-20% glycerol and 0.05% Brij-35 to the KLK12 autoactivation mixture can slow down activation | Homo sapiens |
3.4.21.B43 | Glycerol | adding 10-20% glycerol and 0.05% Brij-35 to the KLK12 autoactivation mixture can slow down activation | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.B43 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.B43 | affinity chromatography and gel filtration | Homo sapiens |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.21.B43 | 37°C, 12.5 mM MES, 75 mM NaCl, after 16 h, 24 h, 48 h, and 72 h only 50%, 35%, 20%, and 10% of the initial activity is detected | Homo sapiens |
3.4.21.B43 | 37°C, in the presence of 10-20% glycerol and 0.05% Brij-35, 72 h, 10% loss of activity | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B43 | alpha2-antiplasmin + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.B43 | Antithrombin III + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.B43 | C1 inhibitor + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.B43 | D-Val-Leu-Lys-thiobenzyl ester + H2O | 100% activity | Homo sapiens | D-Val-Leu-Lys + thiobenzyl alcohol | - |
? | |
3.4.21.B43 | additional information | no cleavage is observed for tert-butyloxycarbonyl-QGR-7-amido-4-methylcoumarin, benzyloxycarbonyl-GGR-7-amido-4-methylcoumarin, L-Pro-Phe-Arg-7-amido-4-methylcoumarin, succinyl-AFK-7-amido-4-methylcoumarin, succinyl-AAPF-7-amido-4-methylcoumarin, succinyl-ALPF-7-amido-4-methylcoumarin, succinyl-LLVY-7-amido-4-methylcoumarin, L-Tyr-7-amido-4-methylcoumarin, and S2586 | Homo sapiens | ? | - |
? | |
3.4.21.B43 | additional information | pro-KLK12 can undergo autoactivation | Homo sapiens | ? | - |
? | |
3.4.21.B43 | protein C inhibitor + H2O | - |
Homo sapiens | ? | - |
? | |
3.4.21.B43 | tert-butyloxycarbonyl-DPR-7-amido-4-methylcoumarin + H2O | 55% activity compared to D-Val-Leu-Lys-thiobenzyl ester | Homo sapiens | tert-butyloxycarbonyl-DPR + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B43 | tert-butyloxycarbonyl-FSR-7-amido-4-methylcoumarin + H2O | 13% activity compared to D-Val-Leu-Lys-thiobenzyl ester | Homo sapiens | tert-butyloxycarbonyl-FSR + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B43 | tert-butyloxycarbonyl-QAR-7-amido-4-methylcoumarin + H2O | 98% activity compared to D-Val-Leu-Lys-thiobenzyl ester | Homo sapiens | tert-butyloxycarbonyl-QAR + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B43 | tert-butyloxycarbonyl-VPR-7-amido-4-methylcoumarin + H2O | 85% activity compared to D-Val-Leu-Lys-thiobenzyl ester | Homo sapiens | tert-butyloxycarbonyl-VPR + 7-amino-4-methylcoumarin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.B43 | kallikrein-related peptidase 12 | - |
Homo sapiens |
3.4.21.B43 | KLK12 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B43 | 0.017 | - |
tert-butyloxycarbonyl-DPR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 0.052 | 2.1 | tert-butyloxycarbonyl-FSR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 0.97 | - |
tert-butyloxycarbonyl-QAR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 8.1 | - |
tert-butyloxycarbonyl-VPR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 10.06 | - |
tert-butyloxycarbonyl-FSR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 12.42 | - |
D-Val-Leu-Lys-thiobenzyl ester | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 17.26 | - |
tert-butyloxycarbonyl-QAR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 25.81 | - |
tert-butyloxycarbonyl-VPR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens | |
3.4.21.B43 | 32.65 | - |
tert-butyloxycarbonyl-DPR-7-amido-4-methylcoumarin | in 0.1 M Tris, 10 mM CaCl2, 0.15 M NaCl, pH 8.0, at 37°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.B43 | 7.5 | 8 | - |
Homo sapiens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.B43 | 5 | 7 | when the pH is decreased to less than 5.0, KLK12 enzyme activity becomes undetectable, KLK12 activity is very sensitive to pH changes in the range 6.5-7.0 (within this range, a decrease of 0.5 pH units results in a more than 60% reduction in activity) | Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.4.21.B43 | 0.011 | - |
- |
Homo sapiens | 3,4-dichloroisocoumarin | |
3.4.21.B43 | 0.28 | - |
- |
Homo sapiens | benzamidine |