EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | D-fructose | slightly stimulating | Priestia megaterium | |
3.2.1.23 | D-glucose | slightly stimulating | Priestia megaterium | |
3.2.1.23 | maltose | slightly stimulating | Priestia megaterium | |
3.2.1.23 | additional information | no activation by melibiose and raffinose | Priestia megaterium | |
3.2.1.23 | sucrose | slightly stimulating | Priestia megaterium |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.23 | non-specific enzyme stabilization by amino acids, overview | Priestia megaterium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | Cs+ | stimulates | Priestia megaterium | |
3.2.1.23 | K+ | stimulates | Priestia megaterium | |
3.2.1.23 | Li+ | stimulates | Priestia megaterium | |
3.2.1.23 | Mn2+ | stimulates | Priestia megaterium | |
3.2.1.23 | Na+ | stimulates | Priestia megaterium | |
3.2.1.23 | NH4+ | stimulates | Priestia megaterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Priestia megaterium | - |
inducible enzyme | - |
3.2.1.23 | Priestia megaterium KM / ATCC 13632 | - |
inducible enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | native enzyme by MnSO4 or streptomycin precipitation, ammonium sulfate fractionation, and starch column electrophoresis | Priestia megaterium |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.23 | -20°C, crude enzyme extract, 24 h, fully stable, loss of 49% activity after 12 days, partially purified enzyme is stable for 60 days | Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Priestia megaterium | 2-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Priestia megaterium KM / ATCC 13632 | 2-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | - |
Priestia megaterium | beta-D-glucose + beta-D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | - |
Priestia megaterium KM / ATCC 13632 | beta-D-glucose + beta-D-galactose | - |
? | |
3.2.1.23 | additional information | the enzyme hydrolyzes methyl-, butyl-, and phenyl-beta-D-galactosides, no activity with thiomethyl-and thiophenyl-beta-D-galactosides or with methyl-beta-L-arabinoside | Priestia megaterium | ? | - |
? | |
3.2.1.23 | additional information | the enzyme hydrolyzes methyl-, butyl-, and phenyl-beta-D-galactosides, no activity with thiomethyl-and thiophenyl-beta-D-galactosides or with methyl-beta-L-arabinoside | Priestia megaterium KM / ATCC 13632 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 40 | - |
assay at | Priestia megaterium |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 55 | - |
5 min, loss of 42% activity in 0.5 M K2HPO4 buffer, no activity loss in 1.5 M K2HP04 | Priestia megaterium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 7.3 | 8 | - |
Priestia megaterium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 6 | 9 | pH profile | Priestia megaterium |