EC Number | Application | Comment | Organism |
---|---|---|---|
4.4.1.5 | additional information | Pseudomonas aeruginosa possesses GlxI enzymes from two metal activation classes. The gloA1 and gloA2 genes encode non-Zn2+ dependent glyoxalase I enzymes and the gloA3 gene encodes a Zn2+-dependent homolog | Pseudomonas aeruginosa PAO1 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.4.1.5 | gloA2 and gloA3 ligated into vector pET22b, overexpression in Escherichia coli BL21(gammaDE3) | Pseudomonas aeruginosa PAO1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | 1,10-phenanthroline | decreases GloA3 activity | Pseudomonas aeruginosa PAO1 | |
4.4.1.5 | dipicolinic acid | greatest loss of GloA3 activity | Pseudomonas aeruginosa PAO1 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.5 | Cd2+ | reactivates GloA3 by 87% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
4.4.1.5 | Co2+ | increases GloA2 activity, hyper-reactivates GloA3 by 115% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
4.4.1.5 | Mg2+ | reactivates GloA3 by 26% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
4.4.1.5 | Mn2+ | hyper-reactivates GloA3 by 146% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
4.4.1.5 | Ni2+ | increases GloA2 activity, hyper-reactivates GloA3 by 146% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 | |
4.4.1.5 | Zn2+ | does not increase GloA2 activity, GloA3 contains Zn2+, reactivates GloA3 by 76% after treatment with dipicolinic acid | Pseudomonas aeruginosa PAO1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.5 | Pseudomonas aeruginosa PAO1 | Q9HU72 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.5 | GloA2 and GloA3, by gel filtration | Pseudomonas aeruginosa PAO1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | glutathione + methylglyoxal | - |
Pseudomonas aeruginosa PAO1 | S-D-lactoylglutathione | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.5 | dimer | GloA2 and GloA3, gel filtration | Pseudomonas aeruginosa PAO1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.5 | GloA1 | - |
Pseudomonas aeruginosa PAO1 |
4.4.1.5 | GloA2 | - |
Pseudomonas aeruginosa PAO1 |
4.4.1.5 | GloA3 | - |
Pseudomonas aeruginosa PAO1 |
4.4.1.5 | GLXI | - |
Pseudomonas aeruginosa PAO1 |
4.4.1.5 | glyoxalase I | - |
Pseudomonas aeruginosa PAO1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 6.6 | - |
- |
Pseudomonas aeruginosa PAO1 |