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Literature summary extracted from

  • Demidkina, T.V.; Faleev, N.G.; Papisova, A.I.; Bazhulina, N.P.; Kulikova, V.V.; Gollnick, P.D.; Phillips, R.S.
    Aspartic acid 214 in Citrobacter freundii tyrosine phenol-lyase ensures sufficient C--H-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site (2006), Biochim. Biophys. Acta, 1764, 1268-1276.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.2 expressed in Escherichia coli SVS370 cells Citrobacter freundii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.2 D214A mutant does not catalyze the decomposition of L-Tyr and 3-fluoro-L-Tyr Citrobacter freundii
4.1.99.2 D214N mutant does not catalyze the decomposition of L-Tyr and 3-fluoro-L-Tyr Citrobacter freundii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.2 L-Asp
-
 Citrobacter freundii
4.1.99.2 L-Glu
-
 Citrobacter freundii
4.1.99.2 L-homoserine
-
 Citrobacter freundii
4.1.99.2 L-Met
-
 Citrobacter freundii
4.1.99.2 L-Phe
-
 Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.2 0.1
-
 S-(o-nitrophenyl)-L-Cys wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.1
-
 3-fluoro-L-Tyr wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.2
-
 L-Tyr wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.2
-
 S-benzyl-L-Cys wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 1.32
-
 S-(o-nitrophenyl)-L-Cys mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 1.86
-
 S-(o-nitrophenyl)-L-Cys mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 2
-
 3-chloro-L-Ala wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 3.4
-
 S-methyl-L-Cys wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 5.1
-
 S-benzyl-L-Cys mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 7
-
 S-benzyl-L-Cys mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 11.4
-
 3-chloro-L-Ala mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 13.3
-
 3-chloro-L-Ala mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 13.4
-
 S-methyl-L-Cys mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 50
-
 S-methyl-L-Cys mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.2 K+ activating Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.2 51400
-
 4 * 51400 Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Citrobacter freundii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.2 ammonium sulfate precipitation Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 3-chloro-L-Ala + H2O
-
 Citrobacter freundii ?
-
 ?
4.1.99.2 3-fluoro-L-tyrosine + H2O
-
 Citrobacter freundii 3-fluorophenol + pyruvate + NH3
-
 ?
4.1.99.2 L-tyrosine + H2O
-
 Citrobacter freundii phenol + pyruvate + NH3
-
 ?
4.1.99.2 S-(o-nitrophenyl)-L-Cys + H2O
-
 Citrobacter freundii ? + pyruvate + NH3
-
 ?
4.1.99.2 S-benzyl-L-Cys + H2O
-
 Citrobacter freundii ? + pyruvate + NH3
-
 ?
4.1.99.2 S-methyl-L-Cys + H2O
-
 Citrobacter freundii ? + pyruvate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.2 homotetramer 4 * 51400 Citrobacter freundii

Synonyms

EC Number Synonyms Comment Organism
4.1.99.2 TPL
-
 Citrobacter freundii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.99.2 0.0016
-
 S-methyl-L-Cys mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.0052
-
 S-methyl-L-Cys mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.0065
-
 3-chloro-L-Ala mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.0067
-
 S-benzyl-L-Cys mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.02
-
 3-chloro-L-Ala mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.02
-
 S-benzyl-L-Cys mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.024
-
 S-(o-nitrophenyl)-L-Cys mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.041
-
 S-(o-nitrophenyl)-L-Cys mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.5
-
 S-benzyl-L-Cys wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.7
-
 3-chloro-L-Ala wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 0.9
-
 S-methyl-L-Cys wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 1.4
-
 3-fluoro-L-Tyr wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 3.5
-
 L-Tyr wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 5.1
-
 S-(o-nitrophenyl)-L-Cys wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.2 pyridoxal 5'-phosphate dependent Citrobacter freundii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.99.2 0.73
-
 L-Met wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate Citrobacter freundii
4.1.99.2 0.73
-
 L-Met wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate Citrobacter freundii
4.1.99.2 1.33
-
 L-homoserine wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate Citrobacter freundii
4.1.99.2 1.33
-
 L-homoserine wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate Citrobacter freundii
4.1.99.2 1.7
-
 L-Phe wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate Citrobacter freundii
4.1.99.2 1.7
-
 L-Phe wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate Citrobacter freundii
4.1.99.2 3
-
 L-Asp mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 3.5
-
 L-Asp wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate Citrobacter freundii
4.1.99.2 3.5
-
 L-Asp wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate Citrobacter freundii
4.1.99.2 3.6
-
 L-Asp mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 4.2
-
 L-Met mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 5
-
 L-Glu wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using L-Tyr as a substrate Citrobacter freundii
4.1.99.2 5
-
 L-Glu wild type enzyme, in 50 mM potassium phosphate pH 8.0, at 25°C, using S-(o-nitrophenyl)-L-Cys as a substrate Citrobacter freundii
4.1.99.2 11
-
 L-Met mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 17
-
 L-Phe mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 22
-
 L-Phe mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 66
-
 L-Glu mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 69
-
 L-homoserine mutant enzyme D214N, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 82
-
 L-homoserine mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii
4.1.99.2 100
-
 L-Glu mutant enzyme D214A, in 50 mM potassium phosphate pH 8.0, at 25°C Citrobacter freundii