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Literature summary extracted from
- Expression and characterization of Arabidopsis phospholipase Dgamma 2 (2006), Biochim. Biophys. Acta, 1761, 1450-1458.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | phosphatidylinositol 4,5-bisphosphate | required | Arabidopsis thaliana |  |
| 3.1.4.4 | Triton X-100 | greatly stimulating | Arabidopsis thaliana | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | expression of variant PLDgamma2a in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Triton X-100 | complete loss of activity and prohibition of any stimulation by phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | required | Arabidopsis thaliana | |
| 3.1.4.4 | Ca2+ | required, highest activity in presence of micromolar concentrations | Arabidopsis thaliana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 95600 | - |
x * 95600, calculated | Arabidopsis thaliana |
| 3.1.4.4 | 96000 | - |
x * 96000, calculated | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Arabidopsis thaliana | Q9T051 | isoform PLDgamma2 | - |
| 3.1.4.4 | Arabidopsis thaliana | Q9T053 | isoform PLDgamma1 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | presence of phosphatidylinositol 4,5-bisphosphate and phosphatidylethanol is required | Arabidopsis thaliana | 1,2-diacylglycerophosphate + choline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | ? | x * 95600, calculated | Arabidopsis thaliana |
| 3.1.4.4 | ? | x * 96000, calculated | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 6.5 | 7.5 | - |
Arabidopsis thaliana |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.4.4 | Arabidopsis thaliana | calculated | - |
8.3 |
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