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Literature summary extracted from

  • Gora, S.; Lambeau, G.; Bollinger, J.G.; Gelb, M.; Ninio, E.; Karabina, S.
    The proinflammatory mediator Platelet Activating Factor is an effective substrate for human group X secreted phospholipase A2 (2006), Biochim. Biophys. Acta, 1761, 1093-1099.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.4 H48Q site-directed mutagenesis, inactive catalytic site mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine competitive inhibition of platelet activating factor hydrolysis Homo sapiens
3.1.1.4 EDTA 95% inhibition at 5 mM Homo sapiens
3.1.1.4 LY 329722 i.e. 3-(3-aminooxalyl-1-benzyl-2-ethyl-6-methyl-1H-indol-4-yl)propionic acid Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 extracellular the enzyme is secreted Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 phosphatidylcholine + H2O Homo sapiens
-
 1-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.4 platelet activating factor + H2O Homo sapiens hGX sPLA2 hydrolyzes platelet activating factor, which is a reaction catalyzed by the platelet activating factor-acetylhydrolase, EC 3.1.1.47 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 0.14
-
 substrate 1-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine Homo sapiens
3.1.1.4 8.56
-
 substrate 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF or platelet activating factor Homo sapiens 1-hexadecanoyl-glycero-3-phosphoglycerol + acetate
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-glycero-3-phosphocholine + arachidonate
-
 ?
3.1.1.4 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O preferred substrate Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
3.1.1.4 additional information hGX sPLA2 prefers phosphatidylcholine 50fold to 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine as substrate, its hydrolyzing activity towards platelet activating factor rises abruptly when the concentration of the substrate passes through its critical micelle concentration, overview Homo sapiens ?
-
 ?
3.1.1.4 phosphatidylcholine + H2O
-
 Homo sapiens 1-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.4 platelet activating factor + H2O hGX sPLA2 hydrolyzes platelet activating factor, which is a reaction catalyzed by the platelet activating factor-acetylhydrolase, EC 3.1.1.47 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 hGX sPLA2
-
 Homo sapiens
3.1.1.4 More the enzyme is a group X secreted phospholipase A2 Homo sapiens
3.1.1.4 PLA2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 37
-
 assay at Homo sapiens