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Literature summary extracted from

  • Yakovlev, A.A.; Gorokhovatsky, A.Y.; Onufriev, M.V.; Beletsky, I.P.; Gulyaeva, N.V.
    Brain cathepsin B cleaves a caspase substrate (2008), Biochemistry (Moscow), 73, 332-336.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 dithiothreitol the presence of dithiothreitol at concentrations of more than 10 mM significantly increases activity at pH 4.0 Rattus norvegicus
3.4.22.1 reduced glutathione the presence of reduced glutathione at concentrations of more than 10 mM significantly increases activity at pH 4.0 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 acetyl-Asp-Glu-Val-Asp-CHO complete DEVDase activity inhibition in brain cell supernatant Rattus norvegicus
3.4.22.1 acetyl-DEVD-CHO
-
Rattus norvegicus
3.4.22.1 Ca2+ 40% inhibition at 2 mM Rattus norvegicus
3.4.22.1 iodoacetamide concentrations above10 mM significantly decrease activity Rattus norvegicus
3.4.22.1 Mg2+ 50% inhibition at 2 mM Rattus norvegicus
3.4.22.1 Mn2+ 10% inhibition at 8 mM, no inhibition at 2 mM Rattus norvegicus
3.4.22.1 N-ethylmaleimide concentrations above10 mM significantly decrease activity Rattus norvegicus
3.4.22.1 oxidized glutathione concentrations above10 mM significantly decrease activity Rattus norvegicus
3.4.22.1 Zn2+ 80% inhibition at 2 mM Rattus norvegicus
3.4.22.1 [L-3-trans-(propylcarbamoyl)oxirane-2-carbonyl]-L-isoleucyl-L-proline
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.1 cytoplasm in oxidative stress cathepsin B may be released from lysosomes into cytoplasm with lowered pH and cleave cytoplasmic proteins, including caspase-3 substrates Rattus norvegicus 5737
-
3.4.22.1 lysosome
-
Rattus norvegicus 5764
-
3.4.22.1 lysosome in oxidative stress cathepsin B may be released from lysosomes into cytoplasm with lowered pH and cleave cytoplasmic proteins, including caspase-3 substrates Rattus norvegicus 5764
-
3.4.22.1 soluble
-
Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 additional information Rattus norvegicus under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1 native enzyme from brain cell supernatant by ammonium sulfate fractionation and gel filtration Rattus norvegicus
3.4.22.1 S-Sepharose Fast Flow column chromatography and Sephadex G-25 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 brain
-
Rattus norvegicus
-
3.4.22.1 neuroblastoma cell
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O a synthetic substrate of caspase-3 and caspase-7 Rattus norvegicus ?
-
?
3.4.22.1 acetyl-DEVD-7-amido-4-methylcoumarin + H2O cleavage occurs at pH 4.0, but not at pH 7.5 Rattus norvegicus acetyl-DEVD + 7-amino-4-methylcoumarin
-
?
3.4.22.1 additional information under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells Rattus norvegicus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.1 CatB
-
Rattus norvegicus
3.4.22.1 DEVDase
-
Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 4
-
-
Rattus norvegicus
3.4.22.1 4
-
with substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.1 3.5 6.5 activity range with substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, about 90% of maximal activity at pH 3.5, maximal activity at pH 4.0, and about 5-10% of maximal activity at pH 6.5, iactive at pH 7.0 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.1 7.5
-
no activity at pH 7.5 Rattus norvegicus