EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.1 | dithiothreitol | the presence of dithiothreitol at concentrations of more than 10 mM significantly increases activity at pH 4.0 | Rattus norvegicus | |
3.4.22.1 | reduced glutathione | the presence of reduced glutathione at concentrations of more than 10 mM significantly increases activity at pH 4.0 | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.1 | acetyl-Asp-Glu-Val-Asp-CHO | complete DEVDase activity inhibition in brain cell supernatant | Rattus norvegicus | |
3.4.22.1 | acetyl-DEVD-CHO | - |
Rattus norvegicus | |
3.4.22.1 | Ca2+ | 40% inhibition at 2 mM | Rattus norvegicus | |
3.4.22.1 | iodoacetamide | concentrations above10 mM significantly decrease activity | Rattus norvegicus | |
3.4.22.1 | Mg2+ | 50% inhibition at 2 mM | Rattus norvegicus | |
3.4.22.1 | Mn2+ | 10% inhibition at 8 mM, no inhibition at 2 mM | Rattus norvegicus | |
3.4.22.1 | N-ethylmaleimide | concentrations above10 mM significantly decrease activity | Rattus norvegicus | |
3.4.22.1 | oxidized glutathione | concentrations above10 mM significantly decrease activity | Rattus norvegicus | |
3.4.22.1 | Zn2+ | 80% inhibition at 2 mM | Rattus norvegicus | |
3.4.22.1 | [L-3-trans-(propylcarbamoyl)oxirane-2-carbonyl]-L-isoleucyl-L-proline | - |
Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.22.1 | cytoplasm | in oxidative stress cathepsin B may be released from lysosomes into cytoplasm with lowered pH and cleave cytoplasmic proteins, including caspase-3 substrates | Rattus norvegicus | 5737 | - |
3.4.22.1 | lysosome | - |
Rattus norvegicus | 5764 | - |
3.4.22.1 | lysosome | in oxidative stress cathepsin B may be released from lysosomes into cytoplasm with lowered pH and cleave cytoplasmic proteins, including caspase-3 substrates | Rattus norvegicus | 5764 | - |
3.4.22.1 | soluble | - |
Rattus norvegicus | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.1 | additional information | Rattus norvegicus | under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.1 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.1 | native enzyme from brain cell supernatant by ammonium sulfate fractionation and gel filtration | Rattus norvegicus |
3.4.22.1 | S-Sepharose Fast Flow column chromatography and Sephadex G-25 gel filtration | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.1 | brain | - |
Rattus norvegicus | - |
3.4.22.1 | neuroblastoma cell | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.1 | acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O | a synthetic substrate of caspase-3 and caspase-7 | Rattus norvegicus | ? | - |
? | |
3.4.22.1 | acetyl-DEVD-7-amido-4-methylcoumarin + H2O | cleavage occurs at pH 4.0, but not at pH 7.5 | Rattus norvegicus | acetyl-DEVD + 7-amino-4-methylcoumarin | - |
? | |
3.4.22.1 | additional information | under certain conditions, e.g. in hypoxia, cathepsin B participates in cleavage of caspase-3 substrates in brain cells | Rattus norvegicus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.1 | CatB | - |
Rattus norvegicus |
3.4.22.1 | DEVDase | - |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.1 | 4 | - |
- |
Rattus norvegicus |
3.4.22.1 | 4 | - |
with substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin | Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.1 | 3.5 | 6.5 | activity range with substrate acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin, about 90% of maximal activity at pH 3.5, maximal activity at pH 4.0, and about 5-10% of maximal activity at pH 6.5, iactive at pH 7.0 | Rattus norvegicus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.1 | 7.5 | - |
no activity at pH 7.5 | Rattus norvegicus |