Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, Y.; Zhang, M.; Zhang, H.; Lei, J.; Jin, R.; Xu, S.; Bao, J.; Zhang, L.; Wang, H.
    Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase (2006), Biochemistry, 71, S38-S43.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.48 expressed in Escherichia coli strain BL21 (DE3) Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.48 geomycine 0.5 mg/ml Mycobacterium tuberculosis
4.1.1.48 H2O2 0.001% H2O2 inactivates the enzyme almost completely Mycobacterium tuberculosis
4.1.1.48 kanamycin 0.5 mg/ml Mycobacterium tuberculosis
4.1.1.48 methanol enzyme activity is gradually lost in the presence of increasing concentrations of methanol and completely lost at 65% Mycobacterium tuberculosis
4.1.1.48 streptomycin 0.5 mg/ml Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.48 Ca2+ significantly affects activity, the optimal concentration is about 0.4-2.0 mM Mycobacterium tuberculosis
4.1.1.48 Mg2+ significantly affects activity, the optimal concentration is about 0.4-2.0 mM Mycobacterium tuberculosis
4.1.1.48 Mn2+ significantly affects activity, the optimal concentration is about 0.4-2.0 mM Mycobacterium tuberculosis
4.1.1.48 Na+ significantly affects activity, the optimal concentration is about 0.4-2.0 mM Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.48 28000
-
 SDS-PAGE, recombinant enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Mycobacterium tuberculosis
-
-
-
4.1.1.48 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.48 Ni-NTA His-binding resin affinity chromatography Mycobacterium tuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.48 culture condition:oleate-grown cell 10% (v/v) oleate-albumin-dextrose complex Mycobacterium tuberculosis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.48 0.004
-
 recombinant enzyme, at 37°C, pH 7.0 Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
4.1.1.48 -70°C, 5 mM Tris-HCl (pH 7.0), at least 2 months, remains stable Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Mycobacterium tuberculosis 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Mycobacterium tuberculosis H37Rv 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.48 IGPS
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.48 37
-
 in 5 mM Tris-HCl buffer Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.48 40 50 little loss of activity after a 10 min heating in a water bath at temperatures from 40 to 50°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.48 7
-
 in 5 mM Tris-HCl buffer Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.48 5.5 8 in 5 mM Tris-HCl buffer Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.48 5
-
 slight activity at pH 5.0 Mycobacterium tuberculosis
4.1.1.48 8
-
 almost no activity at pH 8.0 Mycobacterium tuberculosis