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Literature summary extracted from

  • Lindner, H.A.; Alary, A.; Wilke, M.; Sulea, T.
    Probing the acyl-binding pocket of aminoacylase-1 (2008), Biochemistry, 47, 4266-4275.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.14 I177A 2.7fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 I177A 3.5fold increased KM-value compared to the wildtype with N-hexanoyl-L-methionine moiety Homo sapiens
3.5.1.14 I177L 4fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 I177L 5.2fold increased KM-value compared to the wildtype with N-hexanoyl-L-methionine moiety Homo sapiens
3.5.1.14 L372A 2fold decreased KM-value compared to the wildtype with N-isopentanoyl-L-methionine Homo sapiens
3.5.1.14 L372G 10fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 L372I 1,7fold increased KM-value compared to the wildtype with N-isopentanoyl-L-methionine moiety Homo sapiens
3.5.1.14 L372I 3.1fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 L372V 6.4fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 L372V 7.9fold decreased KM-value compared to the wildtype with N-isopentanoyl-L-methionine moiety Homo sapiens
3.5.1.14 T347F 1.8fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 T347G 6.5fold increased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 T347S 1.1fold decreased KM-value compared to the wildtype with N-acetyl-L-methionine moiety Homo sapiens
3.5.1.14 T347S 5.3fold decreased KM-value compared to the wildtype with N-cyclohexanoyl-L-methionine moiety Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.01
-
 N-hexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.03
-
 N-pentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.19
-
 N-pentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.31
-
 N-cyclohexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.31
-
 N-butanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.34
-
 N-propionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.38
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-methionine substrates by Wild-Type porcine Acy1 Sus scrofa
3.5.1.14 0.4
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 0.41
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1 Homo sapiens
3.5.1.14 0.43
-
 N-acetyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.54
-
 N-isopentanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
3.5.1.14 0.55
-
 N-propionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.64
-
 N-cyclopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.73
-
 N-butanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.76
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant Homo sapiens
3.5.1.14 0.81
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-methionine substrates by wild-type human Acy1 Homo sapiens
3.5.1.14 0.9
-
 N-isopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 1.01
-
 N-hexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 1.14
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
3.5.1.14 1.17
-
 N-cyclohexanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 1.35
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
3.5.1.14 1.63
-
 N-benzoyl-L-methionine hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1 Homo sapiens
3.5.1.14 1.72
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
3.5.1.14 2.1
-
 N-isopentanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant Homo sapiens
3.5.1.14 2.72
-
 N-acetyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 2.76
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
3.5.1.14 2.78
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant Homo sapiens
3.5.1.14 3.56
-
 N-hexanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
3.5.1.14 3.71
-
 N-benzoyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1 Sus scrofa
3.5.1.14 4.27
-
 N-isopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 4.32
-
 N-acetyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant Homo sapiens
3.5.1.14 5.22
-
 N-hexanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
3.5.1.14 6.23
-
 N-cyclohexanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 7.18
-
 N-cyclopentanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 7.29
-
 N-isopentanoyl-L-methionine hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
3.5.1.14 15.3
-
 N-isobutanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 16.8
-
 N-formyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 35.4
-
 N-formyl-Lmethionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 58.5
-
 N-isobutanoyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 63
-
 N-acetyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1 Homo sapiens
3.5.1.14 70
-
 N-acetyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by wild-type human Acy1 Homo sapiens
3.5.1.14 102
-
 N-acetyl-L-glutamate hydrolysis of N-acetyl-L-glutamate substrates by Wild-Type porcine Acy1 Sus scrofa
3.5.1.14 168
-
 N-2,2-dimethylpropionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 585
-
 N-2,2-dimethylpropionyl-L-methionine hydrolysis of N-acyl-L-methionine substrates by pAcy1 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Homo sapiens Q03154
-
-
3.5.1.14 Sus scrofa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-2,2-dimethylpropionyl-L-methionine + H2O
-
 Sus scrofa 2,2-dimethyl-propionate + L-methionine
-
 ?
3.5.1.14 N-2,2-dimethylpropionyl-L-methionine + H2O
-
 Homo sapiens 2,2-dimethyl-propionate + L-methionine
-
 ?
3.5.1.14 N-acetyl-L-glutamate + H2O
-
 Sus scrofa acetate + L-glutamate
-
 ?
3.5.1.14 N-acetyl-L-glutamate + H2O
-
 Homo sapiens acetate + L-glutamate
-
 ?
3.5.1.14 N-acetyl-L-methionine + H2O
-
 Homo sapiens acetate + L-methionine
-
 ?
3.5.1.14 N-benzoyl-L-glutamate + H2O
-
 Sus scrofa benzoate + L-glutamate
-
 ?
3.5.1.14 N-benzoyl-L-glutamate + H2O
-
 Homo sapiens benzoate + L-glutamate
-
 ?
3.5.1.14 N-benzoyl-L-methionine + H2O
-
 Sus scrofa benzoate + L-methionine
-
 ?
3.5.1.14 N-benzoyl-L-methionine + H2O
-
 Homo sapiens benzoate + L-methionine
-
 ?
3.5.1.14 N-butanoyl-L-methionine + H2O
-
 Sus scrofa butanoate + L-methionine
-
 ?
3.5.1.14 N-butanoyl-L-methionine + H2O
-
 Homo sapiens butanoate + L-methionine
-
 ?
3.5.1.14 N-cyclohexanoyl-L-methionine + H2O
-
 Homo sapiens hexanoate + L-methionine
-
 ?
3.5.1.14 N-cyclohexanoyl-L-methionine + H2O
-
 Sus scrofa cyclohexanecarboxylate + L-methionine
-
 ?
3.5.1.14 N-cyclopentanoyl-L-methionine + H2O
-
 Homo sapiens pentanoate + L-methionine
-
 ?
3.5.1.14 N-cyclopentanoyl-L-methionine + H2O
-
 Sus scrofa cyclopentanecarboxylate + L-methionine
-
 ?
3.5.1.14 N-formyl-L-methionine + H2O
-
 Homo sapiens formate + L-methionine
-
 ?
3.5.1.14 N-formyl-Lmethionine + H2O
-
 Sus scrofa formate + L-methionine
-
 ?
3.5.1.14 N-hexanoyl-L-methionine + H2O
-
 Sus scrofa hexanoate + L-methionine
-
 ?
3.5.1.14 N-hexanoyl-L-methionine + H2O
-
 Homo sapiens hexanoate + L-methionine
-
 ?
3.5.1.14 N-isobutanoyl-L-methionine + H2O
-
 Sus scrofa isobutanoate + L-methionine
-
 ?
3.5.1.14 N-isobutanoyl-L-methionine + H2O
-
 Homo sapiens isobutanoate + L-methionine
-
 ?
3.5.1.14 N-isopentanoyl-L-methionine + H2O
-
 Sus scrofa isopentanoate + L-methionine
-
 ?
3.5.1.14 N-isopentanoyl-L-methionine + H2O
-
 Homo sapiens isopentanoate + L-methionine
-
 ?
3.5.1.14 N-pentanoyl-L-methionine + H2O
-
 Sus scrofa pentanoate + L-methionine
-
 ?
3.5.1.14 N-pentanoyl-L-methionine + H2O
-
 Homo sapiens pentanoate + L-methionine
-
 ?
3.5.1.14 N-propionyl-L-methionine + H2O
-
 Sus scrofa propionate + L-methionine
-
 ?
3.5.1.14 N-propionyl-L-methionine + H2O
-
 Homo sapiens propionate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.14 hAcy1
-
 Homo sapiens
3.5.1.14 M20
-
 Sus scrofa
3.5.1.14 M20
-
 Homo sapiens
3.5.1.14 pAcy1
-
 Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.14 0.0015
-
 N-2,2-dimethylpropionyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.0015
-
 N-2,2-dimethylpropionyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.00623
-
 N-cyclohexanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.0101
-
 N-benzoyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1 Homo sapiens
3.5.1.14 0.052 2.1 N-hexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
3.5.1.14 0.105
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.115
-
 N-benzoyl-L-glutamate kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1 Sus scrofa
3.5.1.14 0.129
-
 N-cyclohexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 0.145
-
 N-cyclohexanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 0.158
-
 N-benzoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.34
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
3.5.1.14 0.529
-
 N-isobutanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 0.694
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 1.2
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347F-mutant Homo sapiens
3.5.1.14 1.3
-
 N-benzoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 2.01
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
3.5.1.14 3.3
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant Homo sapiens
3.5.1.14 4.3
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 4.49
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372I-mutant Homo sapiens
3.5.1.14 5.36
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372A-mutant Homo sapiens
3.5.1.14 6.08
-
 N-isopentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 6.08
-
 N-isobutanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 9.51
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
3.5.1.14 11.2
-
 N-cyclopentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 12.1
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372V-mutant Homo sapiens
3.5.1.14 13.3
-
 N-cyclopentanoyl-L-methionine kcat the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 16.1
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 16.2
-
 N-pentanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 16.6
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-L372G-mutant Homo sapiens
3.5.1.14 18.2
-
 N-pentanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 20.1
-
 N-benzoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 25.7
-
 N-propionyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 38.3
-
 N-acetyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 39.4
-
 N-hexanoyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
3.5.1.14 46
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347G-mutant Homo sapiens
3.5.1.14 63.4
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 64.6
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 66.7
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177A-mutant Homo sapiens
3.5.1.14 89.8
-
 N-isobutanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 93.7
-
 N-acetyl-L-methionine kcat for the hydrolysis of NAM and selected N-acyl-L-methionine substrates by hAcy1-I177L-mutant Homo sapiens
3.5.1.14 107
-
 N-butanoyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 120
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1-T347S-mutant Homo sapiens
3.5.1.14 134
-
 N-acetyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 139
-
 N-butanoyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 156
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acyl-L-glutamate substrates by hAcy1 Homo sapiens
3.5.1.14 168
-
 N-propionyl-L-methionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 177
-
 N-formyl-L-methionine kcat for the hydrolysis of N-acyl-L-methionine substrates by hAcy1 Homo sapiens
3.5.1.14 200
-
 N-formyl-Lmethionine kcat for the hydrolysis of N-acetyl-L-methionine substrates by pAcy1 Sus scrofa
3.5.1.14 207
-
 N-acetyl-L-glutamate kcat for the hydrolysis of N-acetyl-L-glutamate substrates by pAcy1 Sus scrofa