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Literature summary extracted from

  • Maurus, R.; Begum, A.; Williams, L.K.; Fredriksen, J.R.; Zhang, R.; Withers, S.G.; Brayer, G.D.
    Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity (2008), Biochemistry, 47, 3332-3344.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.1 Br- greater affinity for the enzyme than chloride and a comparable relative activity Homo sapiens
3.2.1.1 Cl- allosteric activation. The enzyme is essentially inactive in the absence of chloride ion Homo sapiens
3.2.1.1 ClO3- activates, optimal concentration is 0.2 mM Homo sapiens
3.2.1.1 HCOO- activates, optimal concentration is 1 mM Homo sapiens
3.2.1.1 I- lower affinity for the enzyme than chloride and higher relative activity Homo sapiens
3.2.1.1 N3- activates, optimal concentration is 1 mM Homo sapiens
3.2.1.1 NO2- activates, optimal concentration is 1 mM Homo sapiens
3.2.1.1 NO3- activates, optimal concentration is 0.1 mM Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.1 recombinant wild-type and N298S variant HPAs are crystallized using the hanging drop vapor diffusion method Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 N298S variant of the enzyme has an approximate 200fold reduction in affinity for chloride ion Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 0.45
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.1 mM NO3- Homo sapiens
3.2.1.1 0.57
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens
3.2.1.1 0.58
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.2 mM ClO3- Homo sapiens
3.2.1.1 0.58
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM NO3- Homo sapiens
3.2.1.1 0.69
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens
3.2.1.1 0.76
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Cl- Homo sapiens
3.2.1.1 1.2
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Br- Homo sapiens
3.2.1.1 1.3
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM I- Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Homo sapiens P04746
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 2-chloro-4-nitrophenyl alpha-maltotrioside + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 1.9
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Br- Homo sapiens
3.2.1.1 2
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.2 mM ClO3- Homo sapiens
3.2.1.1 2.1
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM I- Homo sapiens
3.2.1.1 2.2
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.1 mM NO3- Homo sapiens
3.2.1.1 2.4
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Cl- Homo sapiens
3.2.1.1 3.5
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM NO2- Homo sapiens
3.2.1.1 4.4
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens
3.2.1.1 15.8
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens