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Literature summary extracted from

  • Kolappan, S.; Zwahlen, J.; Zhou, R.; Truglio, J.J.; Tonge, P.J.; Kisker, C.
    Lysine 190 is the catalytic base in MenF, the menaquinone-specific isochorismate synthase from Escherichia coli: implications for an enzyme family (2007), Biochemistry, 46, 946-953.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.4.2 at 2.5 A resolution Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
5.4.4.2 A344T no catalytic activity Escherichia coli
5.4.4.2 E240Q no catalytic activity Escherichia coli
5.4.4.2 K90A no catalytic activity Escherichia coli
5.4.4.2 L255A 50% increase in Km value, 90% decrease in kcat value Escherichia coli
5.4.4.2 R387A no catalytic activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.4.2 0.192
-
 chorismate wild-type, pH 7.5, 37°C Escherichia coli
5.4.4.2 0.319
-
 chorismate mutant L255A, pH 7.5, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.4.2 Mg2+ required Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.4.4.2 Escherichia coli P38051 menaquinone-specific enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.4.2 chorismate = isochorismate K90 is the base that activates water for nucleophilic attack at the chorismate C2 carbon Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.4.2 Chorismate
-
 Escherichia coli Isochorismate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.4.2 MenF
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.4.2 0.031
-
 chorismate mutant L255A, pH 7.5, 37°C Escherichia coli
5.4.4.2 0.35
-
 chorismate wild-type, pH 7.5, 37°C Escherichia coli