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Literature summary extracted from

  • Frase, H.; Lee, I.
    Peptidyl boronates inhibit Salmonella enterica serovar Typhimurium Lon protease by a competitive ATP-dependent mechanism (2007), Biochemistry, 46, 6647-6657.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.21.53 medicine lon is a target in the development of antibiotics Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.53 recombinant wild-type and mutant lon overexpressed in Escherichia coli BL21 (DE3), using plasmids pHF020 and pHF031, respectively Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.53 S680A displays both intrinsic and peptide-stimulated ATP hydrolysis activity comparable to that of the wild-type enzyme but is unable to catalyze peptide bond hydrolysis. Active site serine is required for interaction of inhibitor with lon Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.53 dansyl-YRGIT-Abu not time-dependent inhibitor of peptide hydrolysis activity of lon Salmonella enterica subsp. enterica serovar Typhimurium
3.4.21.53 dansyl-YRGIT-Abu-B(OH)2 potent time-dependent inhibitor of peptide hydrolysis activity of lon, inhibition requires the binding but not hydrolysis of ATP, is an alternative peptidyl boronate inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
3.4.21.53 MG262 potent time-dependent inhibitor of peptide hydrolysis activity of lon, inhibition requires the binding but not hydrolysis of ATP Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.53 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.53
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.53 YRGIT-Abu-SGRQK(Bz) + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.53 lon protease
-
 Salmonella enterica subsp. enterica serovar Typhimurium