Literature summary extracted from
Bender, R.P.; Ham, A.J.; Osheroff, N.
Quinone-induced enhancement of DNA cleavage by human topoisomerase IIalpha: adduction of cysteine residues 392 and 405 (2007), Biochemistry, 46, 2856-2864.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.6.2.2 |
C170A |
potential site of quinone adduction. Mutant does not show reduced sensitivity to benzoquinone |
Homo sapiens |
5.6.2.2 |
C392A |
potential site of quinone adduction. Mutant shows 50% reduced sensitivity to benzoquinone and displays 2fold faster rates of DNA religation than wild-type. Mutation does not affect the ability of benzoquinone to block the N-terinal gate of the enzyme |
Homo sapiens |
5.6.2.2 |
C405A |
potential site of quinone adduction. Mutant shows 50% reduced sensitivity to benzoquinone and displays 2fold faster rates of DNA religation than wild-type. Mutation does not affect the ability of benzoquinone to block the N-terinal gate of the enzyme |
Homo sapiens |
5.6.2.2 |
C455A |
potential site of quinone adduction. Mutant does not show reduced sensitivity to benzoquinone |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.6.2.2 |
benzoquinone |
inhibits DNA religation and blocks N-terminal gate of the protein by cross-linking its two protomer subunits. Adduction at C392 and C405 of protein is important for its action |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.6.2.2 |
Homo sapiens |
- |
isoform IIalpha |
- |