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Literature summary extracted from

  • Huang, S.H.; Rio, D.C.; Marletta, M.A.
    Ligand binding and inhibition of an oxygen-sensitive soluble guanylate cyclase, Gyc-88E, from Drosophila (2007), Biochemistry, 46, 15115-15122.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.6.1.2 additional information biochemical properties of Gyc-88E are unique for guanylate cyclases, the enzyme possibly functions as an oxygen sensor Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.2 Gyc-88E(1-597) expressed in S2 cells as a polyoma-tagged construct under the control of the metallothionein promoter Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.2 CO forms stable complexes with Gyc-88E(1-597), inhibits 2.9fold Drosophila melanogaster
4.6.1.2 NO forms stable complexes with Gyc-88E(1-597), inhibits 2fold Drosophila melanogaster
4.6.1.2 O2 forms stable complexes with Gyc-88E(1-597), inhibits 3.2fold Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.2 0.66
-
 GTP with 0.273 mM oxygen Drosophila melanogaster
4.6.1.2 0.82
-
 GTP under anaerobic conditions Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.2 Fe2+ Gyc-88E(1-597) is a Fe(II)-O2 complex Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.2 143000
-
 gel filtration Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.2 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.2 Gyc-88E(1-597) purified to more than 95% purity, by gel filtration and on anion-exchange column Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.2 5.6
-
 Fe(II)-unligated Gyc-88E(1-597) Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.2 GTP
-
 Drosophila melanogaster 3',5'-cyclic GMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.2 homodimer 2 x 68000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
4.6.1.2 Gyc-88E
-
 Drosophila melanogaster
4.6.1.2 sGC
-
 Drosophila melanogaster
4.6.1.2 soluble guanylate cyclase
-
 Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.6.1.2 0.051
-
 O2
-
 Drosophila melanogaster