Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luk, L.Y.; Bunn, S.; Liscombe, D.K.; Facchini, P.J.; Tanner, M.E.
    Mechanistic studies on norcoclaurine synthase of benzylisoquinoline alkaloid biosynthesis: an enzymatic Pictet-Spengler reaction (2007), Biochemistry, 46, 10153-10161.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.78 overexpressed in Escherichia coli as a recombinant His-tagged enzyme Thalictrum flavum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.78 0.288
-
 4-hydroxyphenylacetaldehyde
-
 Thalictrum flavum
4.2.1.78 335
-
 (3,4-dihydroxyphenyl)acetaldehyde
-
 Thalictrum flavum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.78 Thalictrum flavum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.78 recombinant His-tagged enzyme Thalictrum flavum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.78 3-(2-aminoethyl)phenol + 4-hydroxyphenylacetaldehyde
-
 Thalictrum flavum ?
-
 ?
4.2.1.78 4-(2-aminoethyl)benzene-1,2-diol + (3,4-dihydroxyphenyl)acetaldehyde
-
 Thalictrum flavum ?
-
 ?
4.2.1.78 4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
-
 Thalictrum flavum (S)-Norcoclaurine + H2O
-
 ?
4.2.1.78 5-(2-aminoethyl)-2-methoxyphenol + 4-hydroxyphenylacetaldehyde
-
 Thalictrum flavum ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.78 6
-
 4-hydroxyphenylacetaldehyde cosubstrate: 3-(2-aminoethyl)phenol Thalictrum flavum
4.2.1.78 6
-
 3-(2-aminoethyl)phenol cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol Thalictrum flavum
4.2.1.78 6
-
 5-(2-aminoethyl)-2-methoxyphenol cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol Thalictrum flavum
4.2.1.78 6
-
 4-hydroxyphenylacetaldehyde cosubstrate: 5-(2-aminoethyl)-2-methoxyphenol Thalictrum flavum
4.2.1.78 6.4
-
 4-hydroxyphenylacetaldehyde cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol Thalictrum flavum
4.2.1.78 6.4
-
 4-(2-Aminoethyl)benzene-1,2-diol cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol Thalictrum flavum
4.2.1.78 7
-
 4-(2-Aminoethyl)benzene-1,2-diol cosubstrate: (3,4-dihydroxyphenyl)acetaldehyde Thalictrum flavum
4.2.1.78 7
-
 (3,4-dihydroxyphenyl)acetaldehyde cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol Thalictrum flavum