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Literature summary extracted from

  • Champagne, K.S.; Piscitelli, E.; Francklyn, C.S.
    Substrate recognition by the hetero-octameric ATP phosphoribosyltransferase from Lactococcus lactis (2006), Biochemistry, 45, 14933-14943.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.17 D155A slight increase in kcat, 3.9fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 1.7fold increase in Km-value for ATP Lactococcus lactis
2.4.2.17 K50A 2.3fold increase in kcat, 51fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 1.8fold increase in Km-value for ATP Lactococcus lactis
2.4.2.17 K8A 2.5fold decrease in kcat, 4.9fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 3.1fold increase in Km-value for ATP Lactococcus lactis
2.4.2.17 S140A mutant is unstable, kinetic parameters can not be determined Lactococcus lactis
2.4.2.17 T159A 2.2fold decrease in kcat, 280fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 2.5fold decrease in Km-value for ATP Lactococcus lactis
2.4.2.17 T162A 3.2fold decrease in kcat, 49fold increase in KM-value for 5-phospho-alpha-D-ribose 1-diphosphate, 2.6fold decrease in Km-value for ATP Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 AMP competitive Lactococcus lactis
2.4.2.17 histidine noncompetitive Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.17 0.0184
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, wild-type enzyme Lactococcus lactis
2.4.2.17 0.0712
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme D155A Lactococcus lactis
2.4.2.17 0.09
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K8A Lactococcus lactis
2.4.2.17 0.901
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T162A Lactococcus lactis
2.4.2.17 0.94
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A Lactococcus lactis
2.4.2.17 1.05
-
 ATP 25°C, pH 8, mutant enzyme T162A Lactococcus lactis
2.4.2.17 1.07
-
 ATP 25°C, pH 8, mutant enzyme T159A Lactococcus lactis
2.4.2.17 2.7
-
 ATP 25°C, pH 8, wild-type enzyme Lactococcus lactis
2.4.2.17 4.7
-
 ATP 25°C, pH 8, mutant enzyme D155A Lactococcus lactis
2.4.2.17 5
-
 ATP 25°C, pH 8, mutant enzyme K50A Lactococcus lactis
2.4.2.17 5.15
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T159A Lactococcus lactis
2.4.2.17 8.45
-
 ATP 25°C, pH 8, mutant enzyme K8A Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Lactococcus lactis first reaction of histidine biosynthesis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Lactococcus lactis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate first reaction of histidine biosynthesis Lactococcus lactis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.17 octamer four HisGS catalytic subunits related to periplasmic binding proteins and four HisZ regulatory subunits that resemble histidyl-tRNA synthetases Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 ATP-PRT
-
 Lactococcus lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.17 additional information
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A Lactococcus lactis
2.4.2.17 0.84
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T162A Lactococcus lactis
2.4.2.17 1.07
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K8A Lactococcus lactis
2.4.2.17 1.2
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme T159A Lactococcus lactis
2.4.2.17 2.7
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, wild-type enzyme Lactococcus lactis
2.4.2.17 2.83
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme D155A Lactococcus lactis
2.4.2.17 6.16
-
 5-phospho-alpha-D-ribose 1-diphosphate 25°C, pH 8, mutant enzyme K50A Lactococcus lactis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.17 0.0811
-
 histidine 25°C, pH 8 Lactococcus lactis
2.4.2.17 1.44
-
 AMP 25°C, pH 8 Lactococcus lactis