EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.31 | expressed in Escherichia coli | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.31 | (R,S)-2-hydroxy-3-butynoic acid | irreversible inactivation | Pseudomonas putida | |
1.1.99.31 | (S)-1-phenyl-1,2-ethanediol | competitive inhibitor | Pseudomonas putida | |
1.1.99.31 | (S)-2-methoxy-2-phenylacetate | competitive inhibitor | Pseudomonas putida | |
1.1.99.31 | (S)-3-phenyllactate | competitive inhibitor | Pseudomonas putida | |
1.1.99.31 | 2-phenylacetate | competitive inhibitor | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 0.33 | - |
(R,S)-mandelate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 0.4 | - |
(R,S)-indoleglycolate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 0.75 | - |
(R,S)-2-hydroxyoctanoate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 0.9 | - |
(R,S)-3-indolelactate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 2 | - |
DL-3-phenyllactate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 4.3 | - |
(R,S)-2-hydroxyisocaproate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 4.9 | - |
(R,S)-2-hydroxyhexanoate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 9.5 | - |
(R,S)-2-hydroxyisovalerate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 15.3 | - |
(R,S)-2-hydroxyvalerate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 22 | - |
(R,S)-2-hydroxy-3-butynoic acid | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida | |
1.1.99.31 | 32 | - |
(R,S)-2-hydroxybutyrate | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.31 | Pseudomonas putida | P20932 | strain ATCC 12633 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.31 | His6-tagged enzyme, Ni-resin column chromatography and Sephadex G-25 gel filtration | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.31 | (R,S)-2-hydroxy-3-butenoic acid + acceptor | very low binding affinity for MDH | Pseudomonas putida | 2-oxo-3-butenoic acid + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxybutyrate + acceptor | slow substrate | Pseudomonas putida | 2-oxobutyrate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxybutyric acid + acceptor | very low binding affinity for MDH | Pseudomonas putida | 2-oxo-3-butynoic acid + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxyhexanoate + acceptor | - |
Pseudomonas putida | 2-oxohexanoate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxyisocaproate + acceptor | - |
Pseudomonas putida | 2-oxoisocaproate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxyisovalerate + acceptor | - |
Pseudomonas putida | 2-oxo-isovalerate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxyoctanoate + acceptor | slow substrate | Pseudomonas putida | 2-oxooctanoate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-2-hydroxyvalerate + acceptor | - |
Pseudomonas putida | 2-oxovalerate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-3-indolelactate + acceptor | - |
Pseudomonas putida | 3-(1H-indol-2-yl)-2-oxopropanoate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-3-phenyllactate + 2,6-dichlorophenolindophenol | slow substrate | Pseudomonas putida | phenylpyruvate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.31 | (R,S)-indoleglycolate + acceptor | - |
Pseudomonas putida | indol-2-yl(oxo)acetate + reduced acceptor | - |
? | |
1.1.99.31 | (R,S)-mandelate + 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.1.99.31 | (R,S)-mandelate + ferricyanide | - |
Pseudomonas putida | 2-oxo-2-phenylacetate + ferrocyanide | - |
? | |
1.1.99.31 | 2-hydroxy-3-butynoate + acceptor | - |
Pseudomonas putida | 2-oxo-3-butynoate + reduced acceptor | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.31 | MDH | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.31 | FMN | - |
Pseudomonas putida |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.31 | 36 | - |
(R,S)-2-hydroxy-3-butynoic acid | in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C | Pseudomonas putida |