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Literature summary extracted from

  • Richmond, G.S.; Smith, T.K.
    The role and characterization of phospholipase A1 in mediating lysophosphatidylcholine synthesis in Trypanosoma brucei (2007), Biochem. J., 405, 319-329.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.32 additional information TbPLA1 does not require metal cofactors for activity, but it does require interfacial activation prior to catalysis, activation by Triton X-100/glycerophosphatidylcholine mixed micelle surfaces is not specific and does not require the preformation of a specific enzyme-substrate complex to achieve surface binding, activation kinetics and mechanism, overview Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.32 additional information construction of the PLA1 deletion mutant cell line BSF lacking enzyme activity, overview Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 arachidonyl trifluoromethyl ketone analogue
-
 Trypanosoma brucei
3.1.1.32 Ca2+ slight inhibition at 2 mM Trypanosoma brucei
3.1.1.32 Cd2+ complete inhibition at 2 mM Trypanosoma brucei
3.1.1.32 Co2+ 43% inhibition at 2 mM Trypanosoma brucei
3.1.1.32 Cu2+ complete inhibition at 2 mM Trypanosoma brucei
3.1.1.32 di-isopropyl fluorophosphate TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei
3.1.1.32 diethyl-p-nitrophenyl phosphate
-
 Trypanosoma brucei
3.1.1.32 E-600
-
 Trypanosoma brucei
3.1.1.32 Fe2+ complete inhibition at 2 mM Trypanosoma brucei
3.1.1.32 Mn2+ slight inhibition at 2 mM Trypanosoma brucei
3.1.1.32 additional information PLA1 is not affected by Mg2+, Cs2+, and EDTA Trypanosoma brucei
3.1.1.32 Ni2+ 57% inhibition at 2 mM Trypanosoma brucei
3.1.1.32 palmitoyl trifluoromethyl ketone analogue
-
 Trypanosoma brucei
3.1.1.32 PMSF TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei
3.1.1.32 Zn2+ 66% inhibition at 2 mM Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.32 additional information
-
 additional information kinetic analysis of enzyme activation Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.32 additional information TbPLA1 does not require metal co-factors for activity Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 29700
-
 soluble free enzyme, gel filtration Trypanosoma brucei
3.1.1.32 137000
-
 Triton X-100 micelle-bound PLA1, gel filtration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O Trypanosoma brucei
-
 lysophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O Trypanosoma brucei 427
-
 lysophosphocholine + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Trypanosoma brucei Q6KBS7 from BALB/c mouse bloodstream
-
3.1.1.32 Trypanosoma brucei 427 Q6KBS7 from BALB/c mouse bloodstream
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 trypomastigote from insect and bloodstream, the latter enzyme form shows a 3fold higher activity Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 3 8 recombinant enzyme Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O
-
 Trypanosoma brucei lysophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei lysophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Trypanosoma brucei 427 lysophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O TbPLA1 possesses an active-site serine residue, but is relatively insensitive to serine-modifying reagents such as di-isopropyl fluorophosphate and PMSF Trypanosoma brucei 427 lysophosphocholine + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PLA1
-
 Trypanosoma brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.32 22
-
 assay at Trypanosoma brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 7
-
 assay at Trypanosoma brucei