Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Greig, N.; Wyllie, S.; Vickers, T.J.; Fairlamb, A.H.
    Trypanothione-dependent glyoxalase I in Trypanosoma cruzi (2006), Biochem. J., 400, 217-223.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.4.1.5 medicine high degree of similarity between the trypanosomatid and bacterial GLO1 proteins, contrasting substrate specificities of human and trypanosomatid glyoxalase enzymes suggest that the glyoxalase system may be an attractive target for anti-trypanosomal chemotherapy Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 pET15b-GLO1 construct expressed in Escherichia coli BL21(DE3)pLysS cells Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 additional information S-4-bromobenzylglutathione is inactive as inhibitor Trypanosoma cruzi
4.4.1.5 S-4-bromobenzylglutathionylspermidine potent linear competitive inhibitor, selectively inhibits trypanosomatid GLO1 activity Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.008
-
 glutathionylspermidine with methylglyoxal Trypanosoma cruzi
4.4.1.5 0.014
-
 glutathionylspermidine with phenylglyoxal Trypanosoma cruzi
4.4.1.5 0.109
-
 trypanothione with methylglyoxal Trypanosoma cruzi
4.4.1.5 0.207
-
 trypanothione with phenylglyoxal Trypanosoma cruzi
4.4.1.5 1.8
-
 glutathione with methylglyoxal Trypanosoma cruzi
4.4.1.5 1.8
-
 glutathione with phenylglyoxal Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.5 cytosol
-
 Trypanosoma cruzi 5829
-
4.4.1.5 mitochondrion
-
 Trypanosoma cruzi 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Co2+ only marginally less effective than nickel Trypanosoma cruzi
4.4.1.5 Mn2+
-
 Trypanosoma cruzi
4.4.1.5 Ni2+ most effective Trypanosoma cruzi
4.4.1.5 Zn2+
-
 Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 16647
-
 x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi
4.4.1.5 16650
-
 x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi
4.4.1.5 17000
-
 x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi
4.4.1.5 31300
-
 size exclusion chromatography and analytical ultracentrifugation Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 to homogeneity via nickel affinity and anion exchange chromatography Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.5 epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal
-
 Trypanosoma cruzi S-D-lactoylglutathione
-
 ?
4.4.1.5 glutathione + phenylglyoxal
-
 Trypanosoma cruzi S-mandoylglutathione
-
 ?
4.4.1.5 glutathionylspermidine + methylglyoxal most efficient substrate Trypanosoma cruzi ?
-
 ?
4.4.1.5 glutathionylspermidine + phenylglyoxal
-
 Trypanosoma cruzi ?
-
 ?
4.4.1.5 trypanothione + methylglyoxal
-
 Trypanosoma cruzi S,S'-bis((R)-lactoyl)trypanothione
-
 ?
4.4.1.5 trypanothione + phenylglyoxal
-
 Trypanosoma cruzi S-mandoyltrypanothione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer x * 17000, SDS-PAGE or Western blot analysis, x * 16647, predicted, x * 16650, MALDI-TOF-MS analysis Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Glo1
-
 Trypanosoma cruzi
4.4.1.5 glyoxalase I
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 105
-
 glutathionylspermidine with phenylglyoxal Trypanosoma cruzi
4.4.1.5 161
-
 glutathionylspermidine with methylglyoxal Trypanosoma cruzi
4.4.1.5 265
-
 trypanothione with phenylglyoxal Trypanosoma cruzi
4.4.1.5 363
-
 trypanothione with methylglyoxal Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 7
-
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.4.1.5 0.0054
-
 S-4-bromobenzylglutathionylspermidine
-
 Trypanosoma cruzi