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Literature summary extracted from

  • Cheng, Y.C.; Hsueh, C.C.; Lu, S.C.; Liao, T.H.
    Identification of three crucial histidine residues (His115, His132 and His297) in porcine deoxyribonuclease II (2006), Biochem. J., 398, 177-185.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.22.1 H109L extracellular, about 80% of wild-type activity Sus scrofa
3.1.22.1 H115 extracellular, very little catalytic activity that may be rescued by imidazole, correct protein folding Sus scrofa
3.1.22.1 H132L soluble, very little catalytic activity. Protein is improperly folded and degraded via the proteosomal pathway within 24 h Sus scrofa
3.1.22.1 H206L extracellular, about 66% of wild-type activity Sus scrofa
3.1.22.1 H207L extracellular, about 40% of wild-type activity Sus scrofa
3.1.22.1 H274L extracellular, about 45% of wild-type activity Sus scrofa
3.1.22.1 H297L extracellular, very little catalytic activity that may be rescued by imidazole, correct protein folding Sus scrofa
3.1.22.1 H322L extracellular, activity similar to wild-type Sus scrofa
3.1.22.1 H41L extracellular, about 60% of wild-type activity Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.22.1 Sus scrofa O62855
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.22.1 138
-
 mutant H207L, pH 4.7 Sus scrofa
3.1.22.1 172
-
 mutant H274L, pH 4.7 Sus scrofa
3.1.22.1 219
-
 mutant H206L, pH 4.7 Sus scrofa
3.1.22.1 220
-
 mutant H41L, pH 4.7 Sus scrofa
3.1.22.1 287
-
 mutant H109L, pH 4.7 Sus scrofa
3.1.22.1 321
-
 wild-type, pH 4.7 Sus scrofa
3.1.22.1 331
-
 mutant H322L, pH 4.7 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.22.1 calf thymus DNA + H2O
-
 Sus scrofa 3'-phosphooligonucleotides + 5'-hydroxyoligonucleotides
-
 ?