Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, L.C.; Lee, Y.L.; Leu, R.J.; Shaw, J.F.
    Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L1 (2006), Biochem. J., 397, 69-76.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.2 D154A kcat/KM for p-nitrophenyl butyrate is 18% of wild-type value. kcat/KM for lauroyl-CoA is 11% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 29% of wild-type value Escherichia coli
3.1.2.2 G44A kcat/KM for p-nitrophenyl butyrate is 16% of wild-type value. kcat/KM for lauroyl-CoA is 15% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 25% of wild-type value Escherichia coli
3.1.2.2 H157A kcat/KM for p-nitrophenyl butyrate is 0.09% of wild-type value Escherichia coli
3.1.2.2 N73A kcat/KM for p-nitrophenyl butyrate is 41% of wild-type value. kcat/KM for lauroyl-CoA is 19% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 35% of wild-type value Escherichia coli
3.1.2.2 S10A kcat/KM for p-nitrophenyl butyrate is 0.57% of wild-type value. kcat/KM for lauroyl-CoA is 0.6% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 0.06% of wild-type value Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.043
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme D154A Escherichia coli
3.1.2.2 0.044
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 0.059
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme D154A Escherichia coli
3.1.2.2 0.06
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 0.061
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme, S10A Escherichia coli
3.1.2.2 0.075
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 0.085
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 0.127
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme, S10A Escherichia coli
3.1.2.2 0.146
-
 lauroyl-CoA 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 0.174
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 0.61
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme H157A Escherichia coli
3.1.2.2 0.87
-
 p-nitrophenyl butyrate 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 1.1
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 1.55
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme S10A Escherichia coli
3.1.2.2 1.66
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 1.88
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme D154A Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Escherichia coli P0ADA1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 lauroyl-CoA + H2O
-
 Escherichia coli CoA + laurate
-
 ?
3.1.2.2 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester + H2O
-
 Escherichia coli N-carbobenzoxy-L-tyrosine + p-nitrophenol
-
 ?
3.1.2.2 p-nitrophenyl butyrate + H2O
-
 Escherichia coli p-nitrophenol + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.2 TAP
-
 Escherichia coli
3.1.2.2 thioesterase I multifunctional enzyme with thioesterase, esterase, arylesterase, protease and lysophospholipase activities Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.2 additional information
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme D154A Escherichia coli
3.1.2.2 0.0093
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme H157A Escherichia coli
3.1.2.2 0.02
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme, S10A Escherichia coli
3.1.2.2 0.031 0.51 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 0.052 2.1 lauroyl-CoA 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 0.057
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme, S10A Escherichia coli
3.1.2.2 0.16
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme S10A Escherichia coli
3.1.2.2 0.41
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 0.45
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme D154A Escherichia coli
3.1.2.2 0.55
-
 p-nitrophenyl butyrate 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 0.77
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 1.14
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 3.16
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 5.98
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme D154A Escherichia coli
3.1.2.2 6.44
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme D154A Escherichia coli
3.1.2.2 7.71
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme G44A Escherichia coli
3.1.2.2 7.81
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 10.13
-
 lauroyl-CoA 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 11.99
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.1.2.2 15.29
-
 p-nitrophenyl butyrate 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 23.8
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, wild-type enzyme Escherichia coli
3.1.2.2 88.99
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, wild-type enzyme Escherichia coli