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Literature summary extracted from

  • Kanai, A.; Sato, A.; Imoto, J.; Tomita, M.
    Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein (2006), Biochem. J., 393, 373-379.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.148 additional information autoregulates its own translation, RNA stem-loop structure acts as an inhibitory regulator of translation by preventing the binding of its Shine-Dalgarno-like sequence by positioning it in the stem region, addition of Thy1 into the in vitro translation system also inhibits translation Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.148 overexpression of the recombinant C-terminal His6-tagged protein in Escherichia coli strain BL21(DE3) Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.148 Mn2+ 0.5 mM enhances the activity Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.148 29000
-
 x * 29000, sequence analysis, x * 29000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.148 Pyrococcus furiosus Q8U3C9 DSM3638
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148 to near homogeneity, by heat treatment at 85°C for 15 min, followed by Ni2+ column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.148 additional information possesses RNA-binding activity, binds to the stem–loop RNA structure Pyrococcus furiosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 ? x * 29000, sequence analysis, x * 29000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.148 flavin-dependent thymidylate synthase
-
 Pyrococcus furiosus
2.1.1.148 Thy1
-
 Pyrococcus furiosus
2.1.1.148 thymidylate synthase 1
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.148 75
-
 optimum RNA-binding activity Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.148 additional information
-
 no RNA-binding activity at 95°C Pyrococcus furiosus