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Literature summary extracted from

  • Smalley, J.W.; Birss, A.J.; Szmigielski, B.; Potempa, J.
    Sequential action of R- and K-specific gingipains of Porphyromonas gingivalis in the generation of the haem-containing pigment from oxyhemoglobin (2007), Arch. Biochem. Biophys., 465, 44-49.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.47 molecular biology a combination of both R- and K-gingipains is required for pigment production from oxyhemoglobin by Porphyromonas gingivalis since R-gingipain converts oxyhemoglobin into the methemoglobin form which is more susceptible to Kgp degradation for the eventual release of iron(III) protoporphyrin IX and production of the micro-oxo haem dimer Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.37 leupeptin
-
 Porphyromonas gingivalis
3.4.22.47 Z-Phe-Lys-acyloxymethylketone oxyhemoglobin-Kgp interactions resulting in formation of a hemoglobin hemichrome are inhibited by specific inhibitor Z-Phe-Lys-acyloxymethylketone Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.37 oxyhaemoglobin + H2O Porphyromonas gingivalis data indicate direct product formation without the occurrence of intermediates, reaction required for the formation the the my-oxo heam dimer containing black pigment methaemoglobin
-
 ?
3.4.22.37 oxyhaemoglobin + H2O Porphyromonas gingivalis HG66 data indicate direct product formation without the occurrence of intermediates, reaction required for the formation the the my-oxo heam dimer containing black pigment methaemoglobin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
-
-
3.4.22.37 Porphyromonas gingivalis HG66
-
-
-
3.4.22.47 Porphyromonas gingivalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.37
-
 Porphyromonas gingivalis
3.4.22.47 using gel-filtration Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 oxyhaemoglobin + H2O data indicate direct product formation without the occurrence of intermediates, reaction required for the formation the the my-oxo heam dimer containing black pigment Porphyromonas gingivalis methaemoglobin
-
 ?
3.4.22.37 oxyhaemoglobin + H2O data indicate direct product formation without the occurrence of intermediates, reaction required for the formation the the my-oxo heam dimer containing black pigment Porphyromonas gingivalis HG66 methaemoglobin
-
 ?
3.4.22.37 oxyhaemoglobin + H2O data indicate direct product formation without the occurrence of intermediates Porphyromonas gingivalis methaemoglobin + ?
-
 ?
3.4.22.37 oxyhaemoglobin + H2O data indicate direct product formation without the occurrence of intermediates Porphyromonas gingivalis HG66 methaemoglobin + ?
-
 ?
3.4.22.47 methemoglobin + H2O methemoglobin, formed by treatment of oxyHb with either NaNO2 or by pre-incubation with HRgpA, is rapidly degraded by Kgp compared to oxyhemoglobin Porphyromonas gingivalis ?
-
 ?
3.4.22.47 methemoglobin + H2O methemoglobin, formed by treatment of oxyHb with either NaNO2 or by pre-incubation with HRgpA, is rapidly degraded by Kgp compared to oxyhemoglobin Porphyromonas gingivalis HG66 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 Arg-gingipain
-
 Porphyromonas gingivalis
3.4.22.37 HRgpA
-
 Porphyromonas gingivalis
3.4.22.47 KGP
-
 Porphyromonas gingivalis
3.4.22.47 Lys-gingipain
-
 Porphyromonas gingivalis