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Literature summary extracted from
- Crystal structure of the yeast nicotinamidase Pnc1p (2007), Arch. Biochem. Biophys., 461, 66-75.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | expression in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | resolution 2.9 Angstrom | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.19 | 0.21 | - |
nicotinamide | about 3fold higher than with pyrazinamide as substrate | Saccharomyces cerevisiae |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.1.19 | Zn2+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | nicotinamide + H2O | Saccharomyces cerevisiae | - |
NH3 + nicotinic acid | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | Saccharomyces cerevisiae | - |
NH3 + pyrazinoic acid | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.19 | Saccharomyces cerevisiae | P53184 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.19 | immobilized metal affinity chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.19 | 50.2 | - |
- |
Saccharomyces cerevisiae |
| 3.5.1.19 | 59.4 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.19 | nicotinamide + H2O | - |
Saccharomyces cerevisiae | NH3 + nicotinic acid | - |
? | |
| 3.5.1.19 | pyrazinamide + H2O | - |
Saccharomyces cerevisiae | NH3 + pyrazinoic acid | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.19 | nicotinamidase Pnc1p | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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