BRENDA - Enzyme Database

Changes in the free energy profile of glutamate mutase imparted by the mutation of an active site arginine residue to lysine

Patwardhan, A.; Marsh, E.N.; Arch. Biochem. Biophys. 461, 194-199 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.99.1
-
Clostridium cochlearium
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.1
R100K
mutation significantly impairs the ability of enzyme to catalyze the rearrangement of substrate radical to product radical
Clostridium cochlearium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.14
-
L-threo-3-methylaspartate
wild-type, pH 7.0
Clostridium cochlearium
5.4.99.1
9.5
-
L-threo-3-methylaspartate
mutant R100K, pH 7.0
Clostridium cochlearium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.1
Clostridium cochlearium
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
L-threo-3-methylaspartate
-
677821
Clostridium cochlearium
L-glutamate
-
-
-
r
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.073
-
L-threo-3-methylaspartate
mutant R100K, pH 7.0
Clostridium cochlearium
5.4.99.1
5.4
-
L-threo-3-methylaspartate
wild-type, pH 7.0
Clostridium cochlearium
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.1
-
Clostridium cochlearium
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.1
R100K
mutation significantly impairs the ability of enzyme to catalyze the rearrangement of substrate radical to product radical
Clostridium cochlearium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.14
-
L-threo-3-methylaspartate
wild-type, pH 7.0
Clostridium cochlearium
5.4.99.1
9.5
-
L-threo-3-methylaspartate
mutant R100K, pH 7.0
Clostridium cochlearium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.1
L-threo-3-methylaspartate
-
677821
Clostridium cochlearium
L-glutamate
-
-
-
r
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.4.99.1
0.073
-
L-threo-3-methylaspartate
mutant R100K, pH 7.0
Clostridium cochlearium
5.4.99.1
5.4
-
L-threo-3-methylaspartate
wild-type, pH 7.0
Clostridium cochlearium