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Literature summary extracted from

  • Fu, X.T.; Lin, H.; Kim, S.M.
    Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34 (2008), Appl. Microbiol. Biotechnol., 78, 265-273.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.81 2-mercaptoethanol 30% increase in activity Agarivorans albus
3.2.1.81 dithiothreitol 30% increase in activity Agarivorans albus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 Al3+ strong Agarivorans albus
3.2.1.81 Cu2+ strong Agarivorans albus
3.2.1.81 Fe3+ strong Agarivorans albus
3.2.1.81 Mn2+ strong Agarivorans albus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.81 additional information
-
 additional information ratio of Km/kcat value for hydrolysis of neoagarohexaose and neoagarotetraose is 4040 and 810 per s and M, respectively Agarivorans albus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.81 extracellular
-
 Agarivorans albus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 additional information no metal ions required for activity Agarivorans albus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 50000
-
 gel filtration Agarivorans albus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Agarivorans albus
-
 isolated from gut of a turban shell
-
3.2.1.81 Agarivorans albus YKW-34
-
 isolated from gut of a turban shell
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81 from culture supernatant Agarivorans albus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.81 culture fluid
-
 Agarivorans albus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 25.5
-
 40°C, pH 8.0 Agarivorans albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
 Agarivorans albus neoagarobiose + neoagarotetraose about 75% neoagarobiose plus 25% neoagarotetraose ?
3.2.1.81 agarose + H2O
-
 Agarivorans albus YKW-34 neoagarobiose + neoagarotetraose about 75% neoagarobiose plus 25% neoagarotetraose ?
3.2.1.81 additional information no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan Agarivorans albus ?
-
 ?
3.2.1.81 additional information no substrate: alginate, carboxymethyl cellulose, fucoidan, kappa-carrageenan, lambda-carrageenan Agarivorans albus YKW-34 ?
-
 ?
3.2.1.81 neoagarohexaose + H2O
-
 Agarivorans albus neoagarobiose
-
 ?
3.2.1.81 neoagarohexaose + H2O
-
 Agarivorans albus YKW-34 neoagarobiose
-
 ?
3.2.1.81 neoagarotetraose + H2O
-
 Agarivorans albus neoagarobiose
-
 ?
3.2.1.81 neoagarotetraose + H2O
-
 Agarivorans albus YKW-34 neoagarobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 monomer 1 * 50000, SDS-PAGE Agarivorans albus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 40
-
-
 Agarivorans albus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.81 30
-
 90% of maximum activity Agarivorans albus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 50
-
 1 h, 95% residual activity. Inactivation above 50°C Agarivorans albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 8
-
-
 Agarivorans albus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.81 6 9 more than 80% of maximum activity Agarivorans albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.81 6 11
-
 Agarivorans albus