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Literature summary extracted from

  • Kasieczka-Burnecka, M.; Kuc, K.; Kalinowska, H.; Knap, M.; Turkiewicz, M.
    Purification and characterization of two cold-adapted extracellular tannin acyl hydrolases from an Antarctic strain Verticillium sp. P9 (2007), Appl. Microbiol. Biotechnol., 77, 77-89.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 2-mercaptoethanol inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 4-chloromercuribenzoate inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Ag+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 alpha-glutathione inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Cd2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Co2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 CO32- inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Cu2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Fe3+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Hg2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 K+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Mn2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Pb2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Sn2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 sodium dodecyl sulphate inhibits isozyme TAH II Hypocreales sp.
3.1.1.20 Triton X-100 inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Tween 20 inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Tween 60 inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Tween 80 inhibits both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Zn2+ inhibits both isozymes TAH I and TAH II Hypocreales sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 1.05
-
Tannic acid isozyme TAH I, pH 5.5, 20°C Hypocreales sp.
3.1.1.20 1.51
-
Tannic acid isozyme TAH II, pH 5.5, 20°C Hypocreales sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.20 extracellular
-
Hypocreales sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Br- activates both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Mg2+ activates both isozymes TAH I and TAH II Hypocreales sp.
3.1.1.20 Urea activates both isozymes TAH I and TAH II Hypocreales sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 40000
-
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE Hypocreales sp.
3.1.1.20 46000
-
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE Hypocreales sp.
3.1.1.20 155000
-
isozymes TAH I and TAH II, gel filtration Hypocreales sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Hypocreales sp.
-
Antarctic strain P9, two cold-adapted isozymes TAH I and TAH II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 native isozymes TAH I and TAH II 7.9fold and 10.5fold, respectively, to homogeneity Hypocreales sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.20 61.8
-
purified isozyme TAH I Hypocreales sp.
3.1.1.20 82.2
-
purified isozyme TAH II Hypocreales sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 (+/-)-epicatechin-gallate + H2O
-
Hypocreales sp. epicatechin + gallate
-
?
3.1.1.20 (+/-)-epigallocatechin-3-gallate + H2O
-
Hypocreales sp. epigallocatechin + gallate
-
?
3.1.1.20 m-digallate + H2O
-
Hypocreales sp. gallate
-
?
3.1.1.20 additional information tannase cleaves ester bonds between gallic acid and glucose in tannic acid and m-digallic acid ester linkages, named depside bonds, it also hydrolyzes (+)-epicatechin gallate and (+)-epigallocatechin-3-gallate Hypocreales sp. ?
-
?
3.1.1.20 tannic acid + H2O
-
Hypocreales sp. gallate + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE Hypocreales sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 TAH
-
Hypocreales sp.
3.1.1.20 tannin acyl hydrolase
-
Hypocreales sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 20
-
isozyme TAH II Hypocreales sp.
3.1.1.20 25
-
isozyme TAH I Hypocreales sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 additional information
-
temperature profile of the isozymes Hypocreales sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 additional information
-
temperature profile of the isozymes Hypocreales sp.
3.1.1.20 20 30 isozymes TAH I and TAH II, stable Hypocreales sp.
3.1.1.20 40 50 isozymes TAH I and TAH II, loss of 50-80% activity Hypocreales sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5.5
-
isozymes TAH I and TAH II Hypocreales sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.20 4.5 7.5 loss of 10-30% activity of TAH I and TAH II at "5°C and 4°C Hypocreales sp.