EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.20 | 2-mercaptoethanol | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | 4-chloromercuribenzoate | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Ag+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | alpha-glutathione | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Cd2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Co2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | CO32- | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Cu2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Fe3+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Hg2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | K+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Mn2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Pb2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Sn2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | sodium dodecyl sulphate | inhibits isozyme TAH II | Hypocreales sp. | |
3.1.1.20 | Triton X-100 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Tween 20 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Tween 60 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Tween 80 | inhibits both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Zn2+ | inhibits both isozymes TAH I and TAH II | Hypocreales sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.20 | 1.05 | - |
Tannic acid | isozyme TAH I, pH 5.5, 20°C | Hypocreales sp. | |
3.1.1.20 | 1.51 | - |
Tannic acid | isozyme TAH II, pH 5.5, 20°C | Hypocreales sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.20 | extracellular | - |
Hypocreales sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.20 | Br- | activates both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Mg2+ | activates both isozymes TAH I and TAH II | Hypocreales sp. | |
3.1.1.20 | Urea | activates both isozymes TAH I and TAH II | Hypocreales sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 40000 | - |
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE | Hypocreales sp. |
3.1.1.20 | 46000 | - |
x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE | Hypocreales sp. |
3.1.1.20 | 155000 | - |
isozymes TAH I and TAH II, gel filtration | Hypocreales sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.20 | Hypocreales sp. | - |
Antarctic strain P9, two cold-adapted isozymes TAH I and TAH II | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.20 | native isozymes TAH I and TAH II 7.9fold and 10.5fold, respectively, to homogeneity | Hypocreales sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 61.8 | - |
purified isozyme TAH I | Hypocreales sp. |
3.1.1.20 | 82.2 | - |
purified isozyme TAH II | Hypocreales sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.20 | (+/-)-epicatechin-gallate + H2O | - |
Hypocreales sp. | epicatechin + gallate | - |
? | |
3.1.1.20 | (+/-)-epigallocatechin-3-gallate + H2O | - |
Hypocreales sp. | epigallocatechin + gallate | - |
? | |
3.1.1.20 | m-digallate + H2O | - |
Hypocreales sp. | gallate | - |
? | |
3.1.1.20 | additional information | tannase cleaves ester bonds between gallic acid and glucose in tannic acid and m-digallic acid ester linkages, named depside bonds, it also hydrolyzes (+)-epicatechin gallate and (+)-epigallocatechin-3-gallate | Hypocreales sp. | ? | - |
? | |
3.1.1.20 | tannic acid + H2O | - |
Hypocreales sp. | gallate + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.20 | ? | x * 40000, isozyme TAH I, SDS-PAGE, x * 46000, isozyme TAH II, SDS-PAGE | Hypocreales sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.20 | TAH | - |
Hypocreales sp. |
3.1.1.20 | tannin acyl hydrolase | - |
Hypocreales sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 20 | - |
isozyme TAH II | Hypocreales sp. |
3.1.1.20 | 25 | - |
isozyme TAH I | Hypocreales sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | additional information | - |
temperature profile of the isozymes | Hypocreales sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | additional information | - |
temperature profile of the isozymes | Hypocreales sp. |
3.1.1.20 | 20 | 30 | isozymes TAH I and TAH II, stable | Hypocreales sp. |
3.1.1.20 | 40 | 50 | isozymes TAH I and TAH II, loss of 50-80% activity | Hypocreales sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 5.5 | - |
isozymes TAH I and TAH II | Hypocreales sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.20 | 4.5 | 7.5 | loss of 10-30% activity of TAH I and TAH II at "5°C and 4°C | Hypocreales sp. |