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Literature summary extracted from

  • Chuang, H.H.; Lin, F.P.
    New role of C-terminal 30 amino acids on the insoluble chitin hydrolysis in actively engineered chitinase from Vibrio parahaemolyticus (2007), Appl. Microbiol. Biotechnol., 76, 123-133.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 the recombinant VpChiA and its C-terminal truncated G589 mutantVpChiAG589 encoded in 1881 and 1791 bp DNA fragments, respectively, are expressed in Escherichia coli using the pET-20b(+) expression system Vibrio parahaemolyticus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 G598X C-terminal truncated G589 mutantVpChiAG589 shows higher temperature stability than recombinant VpChiA. kcat for 4-methylumbelliferyl-N,N'-diacetylchitobiose is 1.16fold higher than wild-type value, kcat for 4-methylumbelliferyl-N,N',N''-triacetylchitotriose is 1.8fold lower than wild-type value Vibrio parahaemolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Co2+ 1 mM, 22% inhibition of VpChiA, 15% inhibition of mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 Cu2+ 1 mM, 38% inhibition of VpChiA, 81% inhibition of mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 EDTA 10 mM, 21% inhibition of VpChiA, 17% inhibition of mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 Fe2+ 1 mM, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 Fe3+ 1 mM, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 SDS 5%, abolishes the activities of both VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 Triton X-100 5%, 31% inhibition of VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 Urea 4 mM, 96% inhibition of VpChiA and mutant enzyme VpChiAG589 Vibrio parahaemolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 0.00144
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 0.00261
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
3.2.1.14 0.00775
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
3.2.1.14 0.01172
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Vibrio parahaemolyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 (GlcNAc)7 + H2O
-
 Vibrio parahaemolyticus ?
-
 ?
3.2.1.14 4-methylumbelliferyl-N,N',N''-triacetylchitotriose + H2O
-
 Vibrio parahaemolyticus ?
-
 ?
3.2.1.14 4-methylumbelliferyl-N,N'-diacetylchitobiose + H2O
-
 Vibrio parahaemolyticus ?
-
 ?
3.2.1.14 alpha-chitin + H2O
-
 Vibrio parahaemolyticus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 VpChiA
-
 Vibrio parahaemolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40
-
 substrate: 4-methylumbelliferyl-N,N'-diacetylchitobiose or 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 25 50 25°C: about 25% of maximal activity, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589. 50°C: 70% of maximal activity, VpChiA. 90% of maximal activity, C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 30 min, 25% loss of activity of VpChiA and 5% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 80
-
 30 min, 55% loss of activity of VpChiA and 20% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 90
-
 30 min, 70% loss of activity of VpChiA and 60% loss of activity of C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.52
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
3.2.1.14 0.833
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 13.94
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
3.2.1.14 16.16
-
 4-methylumbelliferyl-N,N'-diacetylchitobiose pH 7, 37°C, mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 21
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, VpChiA Vibrio parahaemolyticus
3.2.1.14 68.87
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, mutant enzyme VpChiAG589 Vibrio parahaemolyticus
3.2.1.14 110.6
-
 4-methylumbelliferyl-N,N',N''-triacetylchitotriose pH 7, 37°C, VpChiA Vibrio parahaemolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
 substrate: 4-methylumbelliferyl-N,N'-diacetylchitobiose or 4-methylumbelliferyl-N,N',N''-triacetylchitotriose, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 5 8 pH 5.0: about 90% of maximal activity, pH 8.0: about 60% of maximal activity, VpChiA and C-terminal truncated G589 mutant enzyme VpChiAG589 Vibrio parahaemolyticus