Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Feng, Y.; Duan, C.J.; Pang, H.; Mo, X.C.; Wu, C.F.; Yu, Y.; Hu, Y.L.; Wei, J.; Tang, J.L.; Feng, J.X.
    Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases (2007), Appl. Microbiol. Biotechnol., 75, 319-328.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 overexpression in Escherichia coli uncultured microorganism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 CuCl2 82% inhibition uncultured microorganism
3.2.1.4 EDTA 53% inhibition uncultured microorganism
3.2.1.4 SDS 66% inhibition uncultured microorganism
3.2.1.4 ZnCl2 47% inhibition uncultured microorganism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information Km-value for carboxymethyl cellulose is 16.07 mg/ml uncultured microorganism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 CaCl2 stimulation to 122% of control uncultured microorganism
3.2.1.4 CoCl2 stimulation to 137% of control uncultured microorganism
3.2.1.4 CrCl3 stimulation to 119% of control uncultured microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured microorganism
-
 in rabbit caecum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant enzyme uncultured microorganism

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 uncultured microorganism ?
-
 ?
3.2.1.4 cellopentaose + H2O
-
 uncultured microorganism cellobiose + cellotriose
-
 ?
3.2.1.4 cellotetraose + H2O
-
 uncultured microorganism 2 cellobiose
-
 ?
3.2.1.4 cellotriose + H2O slightly degraded uncultured microorganism D-glucose + cellobiose
-
 ?
3.2.1.4 additional information no hydrolysis of cellobiose uncultured microorganism ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 umcel5G
-
 uncultured microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 55
-
-
 uncultured microorganism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 30 min, less than 10% loss of activity uncultured microorganism
3.2.1.4 55
-
 unstable above uncultured microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6 6.5 substrate: carboxymethyl cellulose uncultured microorganism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 3.5 10 weak activity at pH 10.0 and below pH 3.5, substrate: carboxymethyl cellulose uncultured microorganism
3.2.1.4 4.5 8 pH 4.5: about 60% of maximal activity, pH 8.0: about 40% of maximal activity uncultured microorganism

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 9 enzyme retains more than 90% activity after storage for 24 h at broad pH ranging from pH 5.0 to 9.0 uncultured microorganism