EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.81 | the deduced enzyme protein contains a N-terminal signal peptide of 29 amino acid residues, followed by a 266 amino acid sequence that is homologous to catalytic module of family 16 glycoside hydrolases, a bacterial immunoglobulin group 2-like domain of 52 amino acid residues, two carbohydratebinding modules of family 6 separated from Big-2-like domain by nine times repeated GDDTDP amino acid sequence. Expression in Escherichia coli | Vibrio sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.81 | AlCl3 | complete inhibition | Vibrio sp. | |
3.2.1.81 | CuCl2 | complete inhibition | Vibrio sp. | |
3.2.1.81 | CuSO4 | complete inhibition | Vibrio sp. | |
3.2.1.81 | HgCl2 | complete inhibition | Vibrio sp. | |
3.2.1.81 | Pb(CH3COO)2 | complete inhibition | Vibrio sp. | |
3.2.1.81 | sodium dodecylsulfate | 0.1%, complete inhibition | Vibrio sp. | |
3.2.1.81 | ZnCl2 | complete inhibition | Vibrio sp. | |
3.2.1.81 | ZnSO4 | complete inhibition | Vibrio sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.81 | additional information | - |
additional information | Km value for agarose is 1.1 mg/ml | Vibrio sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.81 | CaCl2 | slight activation | Vibrio sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.81 | 106062 | - |
x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein | Vibrio sp. |
3.2.1.81 | 120000 | - |
x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein | Vibrio sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.81 | Vibrio sp. | Q0KKW9 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.81 | proteolytic modification | sequence contains a signal peptide of 29 amino acids | Vibrio sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.81 | 16.4 | - |
pH 7.5, 40°C | Vibrio sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.81 | agar + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
3.2.1.81 | agarose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? | |
3.2.1.81 | additional information | no substrate: neoagarotetraose, neoagarobiose, kappa-carrageenan | Vibrio sp. | ? | - |
? | |
3.2.1.81 | neoagarohexaose + H2O | poor substrate | Vibrio sp. | ? | - |
? | |
3.2.1.81 | neoagarooctaose + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose + neoagarobiose | - |
? | |
3.2.1.81 | porphyran + H2O | - |
Vibrio sp. | neoagarohexaose + neoagarotetraose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.81 | ? | x * 106062, calculated, x * 120000, SDS-PAGE of recombinant protein | Vibrio sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.81 | beta-agarase-a | - |
Vibrio sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.81 | 40 | - |
- |
Vibrio sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.81 | 37 | - |
stable below | Vibrio sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.81 | 7.5 | - |
- |
Vibrio sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.81 | 5.5 | 11 | stable at 25°C for 24 h | Vibrio sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.81 | Vibrio sp. | calculated | - |
4.1 |