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Literature summary extracted from
- Increasing synthetic performance of penicillin G acylase from Bacillus megaterium by site-directed mutagenesis (2007), Appl. Microbiol. Biotechnol., 74, 1023-1030.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | synthesis | production of 6-aminopenicillanate and 7-amino-desacetoxycephalosporanic acid as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | native and mutant proteins expresses in Bacillus subtilis WB600 | Priestia megaterium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | F145A | alpha chain, no formation of cephalexin, no activity with nitrophenylacetylaminobenzoic acid | Priestia megaterium |
| 3.5.1.11 | F145L | alpha chain, no formation of cephalexin, no activity with nitrophenylacetylaminobenzoic acid | Priestia megaterium |
| 3.5.1.11 | F145Y | alpha chain, slightly increased formation of cephalexin, 32% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
| 3.5.1.11 | V24F | beta chain, no effect on the formation of cephalexin, 11% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
| 3.5.1.11 | Y144R | alpha chain, reduced formation of cephalexin, 72% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
| 3.5.1.11 | Y144R/V24F | Y144R on alpha chain, V24F on beta chain, reduced formation of cephalexin, 4% of nitrophenylacetylaminobenzoic acid hydrolyzing activity | Priestia megaterium |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.11 | extracellular | - |
Priestia megaterium | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | penicillin G + H2O | Priestia megaterium | - |
6-aminopenicillanic acid + phenylacetic acid | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.11 | Priestia megaterium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.11 | native and mutant proteins | Priestia megaterium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.11 | 0.01 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified F145A mutant enzyme, 37°C | Priestia megaterium |
| 3.5.1.11 | 0.04 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified F145L mutant enzyme, 37°C | Priestia megaterium |
| 3.5.1.11 | 0.85 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified Y144R mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
| 3.5.1.11 | 0.904 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified Y144R/V24F mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
| 3.5.1.11 | 1.22 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified Y144R/V24F mutant enzyme, 37°C | Priestia megaterium |
| 3.5.1.11 | 1.48 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified V24F mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
| 3.5.1.11 | 1.49 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified native enzyme, 30°C, pH 7.0 | Priestia megaterium |
| 3.5.1.11 | 1.94 | - |
7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide as substrates, purified F145Y mutant enzyme, 30°C, pH 7.0 | Priestia megaterium |
| 3.5.1.11 | 3.35 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified V24F mutant enzyme, 37°C | Priestia megaterium |
| 3.5.1.11 | 9.73 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified F145Y mutant enzyme, 37°C | Priestia megaterium |
| 3.5.1.11 | 22.1 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified Y144R mutant enzyme, 37°C | Priestia megaterium |
| 3.5.1.11 | 30.6 | - |
nitrophenylacetylaminobenzoic acid as substrate, purified native enzyme, 37°C | Priestia megaterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.11 | 6-nitro-3-(phenylacetamido)benzoic acid + H2O | - |
Priestia megaterium | phenylacetic acid + 5-amino-2-nitrobenzoic acid | - |
? | |
| 3.5.1.11 | 7-amino-desacetoxycephalosporanic acid + D-phenylglycine amide | high reactant concentrations | Priestia megaterium | cephalexin | - |
r | |
| 3.5.1.11 | cephalosporin G + H2O | - |
Priestia megaterium | 7-amino-desacetoxycephalosporanic acid + phenylacetic acid | - |
? | |
| 3.5.1.11 | penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | ? | alpha and beta chain | Priestia megaterium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.11 | BmPGA | - |
Priestia megaterium |
| 3.5.1.11 | penicillin G acylase | - |
Priestia megaterium |
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