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Literature summary extracted from

  • Umemoto, Y.; Onishi, R.; Araki, T.
    Cloning of a novel gene encoding beta -1,3-xylosidase from a marine bacterium, Vibrio sp. strain XY-214, and characterization of the gene product (2008), Appl. Environ. Microbiol., 74, 305-308.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.72 expression in Escherichia coli Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.72 Ag+ more than 90% inhibition Vibrio sp.
3.2.1.72 Cu2+ more than 90% inhibition Vibrio sp.
3.2.1.72 Hg2+ more than 90% inhibition Vibrio sp.
3.2.1.72 Mn2+ more than 90% inhibition Vibrio sp.
3.2.1.72 p-chloromercuribenzoic acid more than 90% inhibition Vibrio sp.
3.2.1.72 Pb2+ more than 90% inhibition Vibrio sp.
3.2.1.72 Zn2+ more than 90% inhibition Vibrio sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.72 0.244
-
 p-nitrophenyl-beta-D-xylopyranoside
-
 Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.72 60000
-
 SDS-PAGE Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.72 Vibrio sp. A9ZND1
-
-
3.2.1.72 Vibrio sp. XY-214 A9ZND1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.72 wild type by DEAE-Toyopearl 650M and MonoQ 5/50 column chromatography, recombinant protein by HiTrap chelating HP column chromatography Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.72 beta -1,4-xylotetraose low activity Vibrio sp. D-xylose
-
 ?
3.2.1.72 beta -1,4-xylotetraose low activity Vibrio sp. XY-214 D-xylose
-
 ?
3.2.1.72 beta-1,3-xylobiose + H2O complete hydrolysis Vibrio sp. D-xylose
-
 ?
3.2.1.72 beta-1,3-xylobiose + H2O complete hydrolysis Vibrio sp. XY-214 D-xylose
-
 ?
3.2.1.72 beta-1,3-xylotetraose + H2O complete hydrolysis Vibrio sp. D-xylose
-
 ?
3.2.1.72 beta-1,3-xylotetraose + H2O complete hydrolysis Vibrio sp. XY-214 D-xylose
-
 ?
3.2.1.72 beta-1,3-xylotriose + H2O complete hydrolysis Vibrio sp. D-xylose
-
 ?
3.2.1.72 beta-1,3-xylotriose + H2O complete hydrolysis Vibrio sp. XY-214 D-xylose
-
 ?
3.2.1.72 beta-1,4-xylobiose low activity Vibrio sp. D-xylose
-
 ?
3.2.1.72 beta-1,4-xylotriose low activity Vibrio sp. D-xylose
-
 ?
3.2.1.72 beta-1,4-xylotriose low activity Vibrio sp. XY-214 D-xylose
-
 ?
3.2.1.72 p-nitrophenyl-beta-D-xylopyranose + H2O no activity on laminarioligosaccharides, beta-1,3-linked D-glucose units, beta-1,3-xylan, beta-1,4-xylan, carboxymethyl cellulose, laminaran, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-Dgalactopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-L-fucopyranoside, p-nitrophenyl-beta-L-arabinofuranoside, p-nitrophenyl-beta-L-arabinopyranoside, p-nitrophenyl-beta-D-glucuronide, and p-nitrophenyl-beta-D-cellobioside Vibrio sp. p-nitrophenol + D-xylopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.72 tetramer 4 * 60000 gel filtration chromatography on a Superdex 200 HR 10/30 column Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.72 beta-1,3-xylosidase A intracellular Vibrio sp.
3.2.1.72 xloA
-
 Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.72 35
-
-
 Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.72 7
-
 with 2 mM p-nitrophenyl-beta-D-xylopyranoside at 37°C Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.72 6 9 retaining more than 80% of the original activity after preincubation in the buffer at various pHs at 4°C for 12 h Vibrio sp.