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Literature summary extracted from
- NADP(H) phosphatase activities of archaeal inositol monophosphatase and eubacterial 3-phosphoadenosine 5-phosphate phosphatase (2007), Appl. Environ. Microbiol., 73, 5447-5452.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.7 | expressed in Escherichia coli K-12 AG1 | Escherichia coli |
| 3.1.3.25 | expressed in Escherichia coli BL21(DE3) cells | Methanocaldococcus jannaschii |
| 3.1.3.25 | expressed in Escherichia coli Origami B(DE3) cells | Archaeoglobus fulgidus |
| 3.1.3.25 | expressed in Escherichia coli strain K-12 AG1 | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.7 | LiCl | - |
Escherichia coli |  |
| 3.1.3.7 | Mg2+ | activity is decreased to approximately 70% in the presence of 10 mM MgCl2 | Escherichia coli | |
| 3.1.3.25 | LiCl | - |
Archaeoglobus fulgidus | |
| 3.1.3.25 | LiCl | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.7 | 0.14 | - |
adenosine 2',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 0.24 | - |
adenosine 3',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 1.17 | - |
D-fructose 1,6-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 2.13 | - |
NADPH | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 2.7 | - |
NADP+ | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.25 | 0.18 | - |
D-myo-Inositol 1-phosphate | - |
Archaeoglobus fulgidus | |
| 3.1.3.25 | 1.02 | - |
D-myo-Inositol 1-phosphate | - |
Methanocaldococcus jannaschii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.7 | Mg2+ | in the presence of 10 mM MgCl2, CysQ shows maximum activities of 3'-PAPase and NADP(H)ase at pH 6.4 and pH 8.0, respectively | Escherichia coli | |
| 3.1.3.25 | Li+ | 127% activity in the presence of 500 mM Li+ | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.7 | 28000 | - |
SDS-PAGE | Escherichia coli |
| 3.1.3.25 | 28000 | - |
SDS-PAGE | Archaeoglobus fulgidus |
| 3.1.3.25 | 28600 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.7 | Escherichia coli | - |
- |
- |
| 3.1.3.25 | Archaeoglobus fulgidus | O30298 | - |
- |
| 3.1.3.25 | Escherichia coli | - |
- |
- |
| 3.1.3.25 | Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.7 | TALON metal affinity resin column chromatography | Escherichia coli |
| 3.1.3.25 | Toyopearl Super Q-650S column chromatography, phenyl-Sepharose column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration | Methanocaldococcus jannaschii |
| 3.1.3.25 | Toyopearl Super Q-650S column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.7 | 2'-AMP + H2O | 10% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| 3.1.3.7 | 3'-AMP + H2O | 84% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| 3.1.3.7 | adenosine 2',5'-bisphosphate + H2O | 829% relative activity compared to NADP+ | Escherichia coli | adenosine 5'-phosphate + phosphate | - |
? | |
| 3.1.3.7 | adenosine 3',5'-bisphosphate + H2O | 607% relative activity compared to NADP+ | Escherichia coli | adenosine 5'-phosphate + phosphate | - |
? | |
| 3.1.3.7 | D-fructose 1,6-bisphosphate + H2O | 122% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| 3.1.3.7 | D-myo-inositol 2-phosphate + H2O | 9% relative activity compared to NADP+ | Escherichia coli | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.7 | D-myo-inositol monophosphate + H2O | 13% relative activity compared to NADP+ | Escherichia coli | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.7 | D-myo-inositol-1,4,5-trisphosphate + H2O | 14% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| 3.1.3.7 | D-myo-inositol-1,4-bisphosphate + H2O | 485% relative activity compared to NADP+ | Escherichia coli | ? | - |
? | |
| 3.1.3.7 | additional information | glycerol phosphate, D-glucose-1-phosphate, D-glucose-6-phosphate, D-fructose-1-phosphate, D-fructose 1,6-bisphosphate, D-fructose-6-phosphate, ADP, 5'-AMP, ATP, NAD+, NMN+, bis-p-nitrophenol phosphate are no substrates | Escherichia coli | ? | - |
? | |
| 3.1.3.7 | NADP+ + H2O | 100% activity | Escherichia coli | NAD+ + phosphate | - |
? | |
| 3.1.3.7 | NADPH + H2O | 145% relative activity compared to NADP+ | Escherichia coli | NADH + phosphate | - |
? | |
| 3.1.3.7 | p-nitrophenyl phosphate | 21% relative activity compared to NADP+ | Escherichia coli | p-nitrophenol + phosphate | - |
? | |
| 3.1.3.25 | 2'-AMP + H2O | - |
Methanocaldococcus jannaschii | adenosine + phosphate | - |
? | |
| 3.1.3.25 | 2'-AMP + H2O | - |
Archaeoglobus fulgidus | adenosine + phosphate | - |
? | |
| 3.1.3.25 | 2'-phosphoadenosine 5'-phosphate + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
| 3.1.3.25 | 2'-phosphoadenosine 5'-phosphate + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.3.25 | D-fructose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-fructose + phosphate | - |
? | |
| 3.1.3.25 | D-fructose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-fructose + phosphate | - |
? | |
| 3.1.3.25 | D-glucose 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-glucose + phosphate | - |
? | |
| 3.1.3.25 | D-glucose 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-glucose + phosphate | - |
? | |
| 3.1.3.25 | D-myo-inositol 1,4-bisphosphate + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
| 3.1.3.25 | D-myo-inositol 1,4-bisphosphate + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.3.25 | D-myo-inositol 1-phosphate + H2O | - |
Escherichia coli | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.25 | D-myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.25 | D-myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.25 | D-myo-inositol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.25 | D-myo-inositol 2-phosphate + H2O | - |
Archaeoglobus fulgidus | D-myo-inositol + phosphate | - |
? | |
| 3.1.3.25 | additional information | D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates | Methanocaldococcus jannaschii | ? | - |
? | |
| 3.1.3.25 | additional information | D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates | Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.3.25 | additional information | the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP | Methanocaldococcus jannaschii | ? | - |
? | |
| 3.1.3.25 | additional information | the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP | Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.3.25 | p-nitrophenyl phosphate + H2O | - |
Methanocaldococcus jannaschii | p-nitrophenol + phosphate | - |
? | |
| 3.1.3.25 | p-nitrophenyl phosphate + H2O | - |
Archaeoglobus fulgidus | p-nitrophenol + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.25 | dimer | - |
Methanocaldococcus jannaschii |
| 3.1.3.25 | dimer | - |
Archaeoglobus fulgidus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.7 | 3'-PAPase | - |
Escherichia coli |
| 3.1.3.7 | 3'-phosphoadenosine 5'-phosphate phosphatase | - |
Escherichia coli |
| 3.1.3.7 | 3-phosphoadenosine 5-phosphate phosphatase | - |
Escherichia coli |
| 3.1.3.7 | CysQ | exhibits also NADP(H) phosphatase and fructose-1,6-bisphosphatase activities | Escherichia coli |
| 3.1.3.25 | AF2372 | exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to inositol monophosphatase activitity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity | Archaeoglobus fulgidus |
| 3.1.3.25 | IMPase | - |
Escherichia coli |
| 3.1.3.25 | IMPase | - |
Methanocaldococcus jannaschii |
| 3.1.3.25 | IMPase | - |
Archaeoglobus fulgidus |
| 3.1.3.25 | inositol phosphatase | - |
Escherichia coli |
| 3.1.3.25 | inositol phosphatase | - |
Methanocaldococcus jannaschii |
| 3.1.3.25 | inositol phosphatase | - |
Archaeoglobus fulgidus |
| 3.1.3.25 | MJ0109 | exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to the inositol monophosphatase activity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity | Methanocaldococcus jannaschii |
| 3.1.3.25 | SuhB | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.7 | 0.7 | - |
NADP+ | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 1.1 | - |
D-fructose 1,6-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 1.45 | - |
NADPH | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 11.4 | - |
adenosine 3',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.7 | 13.2 | - |
adenosine 2',5'-bisphosphate | in 10 mM HEPES pH 7.0 | Escherichia coli | |
| 3.1.3.25 | 4.1 | - |
D-myo-Inositol 1-phosphate | - |
Methanocaldococcus jannaschii | |
| 3.1.3.25 | 28.7 | - |
D-myo-Inositol 1-phosphate | - |
Archaeoglobus fulgidus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.7 | 6.4 | - |
- |
Escherichia coli |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.7 | 8 | - |
the 3'-PAPase activity is decreased to approximately 50% at pH 8.0 | Escherichia coli |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.7 | 0.05 | - |
using adenosine 3',5'-bisphosphate as a substrate | Escherichia coli | LiCl | |
| 3.1.3.7 | 0.15 | - |
using adenosine 2',5'-bisphosphate as a substrate | Escherichia coli | LiCl | |
| 3.1.3.25 | 30 | - |
- |
Escherichia coli | LiCl | |
| 3.1.3.25 | 30 | - |
- |
Archaeoglobus fulgidus | LiCl | |
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