EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.47 | expressed in Escherichia coli strain JM109 | Priestia megaterium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.47 | E170K | mutant is unstable at an alkaline pH (26% residual activity at pH 10-10.5), dissociates into dimers at an alkaline pH | Priestia megaterium |
1.1.1.47 | Q252L | mutant is inactivated at pH values above 9, dissociates into dimers at an alkaline pH | Priestia megaterium |
1.1.1.47 | Q252L/E170K | mutant exhibits increased pH stability (95% residual activity at pH 8-10.5) in the absence of NaCl | Priestia megaterium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 0.15 | - |
NAD+ | apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 0.17 | - |
NAD+ | apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 0.22 | - |
NAD+ | apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 0.33 | - |
NAD+ | apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 7.5 | - |
D-glucose | apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 8 | - |
D-glucose | apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 8.5 | - |
D-glucose | apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 14 | - |
D-glucose | apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.47 | Priestia megaterium | P40288 | - |
- |
1.1.1.47 | Priestia megaterium IGW3 | P40288 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.47 | high-pressure liquid chromatography | Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | D-glucose + NAD+ | - |
Priestia megaterium | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-glucose + NAD+ | - |
Priestia megaterium IGW3 | D-glucono-1,5-lactone + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.47 | tetramer | gel filtration | Priestia megaterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.47 | GlcDH | - |
Priestia megaterium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 317 | - |
D-glucose | apparent value, mutant enzyme Q252L, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 334 | - |
D-glucose | apparent value, mutant enzyme Q252L/E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 352 | - |
D-glucose | apparent value, mutant enzyme E170K, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium | |
1.1.1.47 | 395 | - |
D-glucose | apparent value, wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0), at 25°C | Priestia megaterium |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 7 | 9 | wild type GlcDH shows reversible dissociation-association between inactive monomers and active tetramers when the pH is shifted between 9 and 7 | Priestia megaterium |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | NAD+ | - |
Priestia megaterium | |
1.1.1.47 | NADP+ | - |
Priestia megaterium |