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Literature summary extracted from

  • Saxena, D.; Aouad, S.; Attieh, J.; Saini, H.S.
    Biochemical characterization of chloromethane emission from the wood-rotting fungus Phellinus pomaceus (1998), Appl. Environ. Microbiol., 64, 2831-2835.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.165 S-adenosyl-L-homocysteine 50% inhibition at equimolar concentrations of S-adenosyl-L-homocysteine and S-adenosyl-L-methionine Phellinus pomaceus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.165 0.0045
-
 S-adenosyl-L-methionine pH 6.8, 22°C Phellinus pomaceus
2.1.1.165 0.25
-
 Iodide pH 6.8, 22°C Phellinus pomaceus
2.1.1.165 10
-
 bromide pH 6.8, 22°C Phellinus pomaceus
2.1.1.165 300
-
 chloride pH 6.8, 22°C Phellinus pomaceus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.165 membrane bound to Phellinus pomaceus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.165 S-adenosyl-L-methionine + chloride Phellinus pomaceus the enzyme is responsible for the massive amounts of CH3Cl produced by this fungus S-adenosyl-L-homocysteine + methyl chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.165 Phellinus pomaceus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.165 the difficulty of solubilization of this membrane-bound labile enzyme is the greatest obstacle to its purification Phellinus pomaceus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.165 mycelium the fungus is cultured in undisturbed glucose mycological peptone liquid medium Phellinus pomaceus
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.165 -20°C to 4°C, partially purified enzyme, complete loss of activity overnight, also in the presence of protease inhibitors Phellinus pomaceus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.165 S-adenosyl-L-methionine + bromide Vmax/Km for bromide is 17fold lower than Vmax/Km for iodide Phellinus pomaceus S-adenosyl-L-homocysteine + methyl bromide
-
 ?
2.1.1.165 S-adenosyl-L-methionine + chloride the enzyme is responsible for the massive amounts of CH3Cl produced by this fungus Phellinus pomaceus S-adenosyl-L-homocysteine + methyl chloride
-
 ?
2.1.1.165 S-adenosyl-L-methionine + chloride Vmax/Km for chloride is 709fold lower than Vmax/Km for iodide Phellinus pomaceus S-adenosyl-L-homocysteine + methyl chloride
-
 ?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Phellinus pomaceus S-adenosyl-L-homocysteine + methyl iodide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.165 7 7.2
-
 Phellinus pomaceus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.165 6 8 pH 6.0: about 35% of maximal activity, pH 8.0: about 75% of maximal activity Phellinus pomaceus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.165 S-adenosyl-L-methionine specific for, natural methyl donor Phellinus pomaceus