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Literature summary extracted from

  • Kaur, P.; Lingner, A.; Singh, B.; Boeer, E.; Polajeva, J.; Steinborn, G.; Bode, R.; Gellissen, G.; Satyanarayana, T.; Kunze, G.
    APHO1 from the yeast Arxula adeninivorans encodes an acid phosphatase of broad substrate specificity (2007), Antonie van Leeuwenhoek, 91, 45-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expressed in Arxula adeninivorans strain G1211 Blastobotrys adeninivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.2
-
 phytic acid recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 0.5
-
 3-indoxyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 0.7
-
 pyridoxal 5'-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1.4
-
 ADP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1.4
-
 AMP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1.7
-
 D-glucose 6-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1.8
-
 Sodium diphosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 2.5
-
 p-nitrophenyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 2.5
-
 1-naphthyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 2.5
-
 Glycerol 2-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 52400
-
 6 * 52400, calculated from amino acid sequence Blastobotrys adeninivorans
3.1.3.2 58330
-
 6 * 58330, gel filtration Blastobotrys adeninivorans
3.1.3.2 314400
-
 calculated from amino acid sequence Blastobotrys adeninivorans
3.1.3.2 350000
-
 gel filtration Blastobotrys adeninivorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Blastobotrys adeninivorans
-
-
-
3.1.3.2 Blastobotrys adeninivorans LS3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 hydroxylapatite column chromatography and Superdex 200 gel filtration Blastobotrys adeninivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Blastobotrys adeninivorans 1-naphthol + phosphate
-
 ?
3.1.3.2 1-naphthyl phosphate + H2O
-
 Blastobotrys adeninivorans LS3 1-naphthol + phosphate
-
 ?
3.1.3.2 3-indoxyl phosphate + H2O
-
 Blastobotrys adeninivorans 3-indoxol + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Blastobotrys adeninivorans AMP + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O
-
 Blastobotrys adeninivorans D-glucose + phosphate
-
 ?
3.1.3.2 D-glucose 6-phosphate + H2O
-
 Blastobotrys adeninivorans LS3 D-glucose + phosphate
-
 ?
3.1.3.2 glycerol 2-phosphate + H2O
-
 Blastobotrys adeninivorans glycerol + phosphate
-
 ?
3.1.3.2 glycerol 2-phosphate + H2O
-
 Blastobotrys adeninivorans LS3 glycerol + phosphate
-
 ?
3.1.3.2 additional information no activity with cAMP Blastobotrys adeninivorans ?
-
 ?
3.1.3.2 additional information no activity with cAMP Blastobotrys adeninivorans LS3 ?
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Blastobotrys adeninivorans p-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Blastobotrys adeninivorans LS3 p-nitrophenol + phosphate
-
 ?
3.1.3.2 phytic acid + H2O
-
 Blastobotrys adeninivorans ? + phosphate
-
 ?
3.1.3.2 pyridoxal 5'-phosphate + H2O
-
 Blastobotrys adeninivorans pyridoxal + phosphate
-
 ?
3.1.3.2 sodium diphosphate + H2O
-
 Blastobotrys adeninivorans sodium phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 hexamer 6 * 52400, calculated from amino acid sequence Blastobotrys adeninivorans
3.1.3.2 hexamer 6 * 58330, gel filtration Blastobotrys adeninivorans

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 Apho1p
-
 Blastobotrys adeninivorans
3.1.3.2 orthophosphoric-monoester phosphohydrolase
-
 Blastobotrys adeninivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 60
-
-
 Blastobotrys adeninivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 45 60
-
 Blastobotrys adeninivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 845
-
 AMP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 915
-
 phytic acid recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1056
-
 3-indoxyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1831
-
 Glycerol 2-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 1901
-
 pyridoxal 5'-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 2042
-
 Sodium diphosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 2462
-
 D-glucose 6-phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 2676
-
 ADP recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 5282
-
 1-naphthyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans
3.1.3.2 40850
-
 p-nitrophenyl phosphate recombinant enzyme, in 100 mM sodium acetate buffer (pH 4.5), at 60°C Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.8
-
-
 Blastobotrys adeninivorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 4.2 5.3
-
 Blastobotrys adeninivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.2 Blastobotrys adeninivorans isoelectric focusing
-
 5.13