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Literature summary extracted from

  • Malloy, J.L.; Veldhuizen, R.A.; Thibodeaux, B.A.; O'Callaghan, R.J.; Wright, J.R.
    Pseudomonas aeruginosa protease IV degrades surfactant proteins and inhibits surfactant host defense and biophysical functions (2005), Am. J. Physiol. Lung Cell Mol. Physiol., 288, L409-L418.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.50 30000
-
 x * 30000, about, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.50 additional information Pseudomonas aeruginosa the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview ?
-
 ?
3.4.21.50 additional information Pseudomonas aeruginosa PA103-29 the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Pseudomonas aeruginosa
-
-
-
3.4.21.50 Pseudomonas aeruginosa PA103-29
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.50 native enzyme from strain PA103-29 by ion exchange chromatography and gel filtration, or from strain PAO1-B1 supernatants by ultrafiltration, anion exchange and hydrophobic interaction chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.50 additional information
-
 pathogen-host surface activity analysis, overview Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 additional information the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview Pseudomonas aeruginosa ?
-
 ?
3.4.21.50 additional information the enzyme inhibits four host cell surfactant-associated proteins designated SP-A, SP-B, SP-C, and SP-D, degradation of SPs by protease IV inhibits their ability to enhance bacterial association with alveolar macrophages, overview Pseudomonas aeruginosa PA103-29 ?
-
 ?
3.4.21.50 tosyl-Gly-Pro-Lys-4-nitroanilide + H2O a chromogenic substrate Pseudomonas aeruginosa tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?
3.4.21.50 tosyl-Gly-Pro-Lys-4-nitroanilide + H2O a chromogenic substrate Pseudomonas aeruginosa PA103-29 tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.50 ? x * 30000, about, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.4.21.50 protease IV
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.50 25 37 assay at Pseudomonas aeruginosa