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Literature summary extracted from

  • Katsumi, R.; Koga, Y.; You, D.J.; Matsumura, H.; Takano, K.; Kanaya, S.
    Crystallization and preliminary x-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis (2007), Acta Crystallogr. Sect. F, F63, 126-129.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.30 overproduced in Escherichia coli BL21 (DE3) Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.30 using sitting-drop vapour-diffusion method at 293 K. Native Tk-glycerol kinase crystals appear after a few days using Wizard I solution No. 25 (0.1 M Tris pH 8.5, 0.2 M MgCl2, 30% PEG 400). Diffraction spots sufficient for structural determination at high resolution are not obtained when a crystal of Tk-glycerol kinase is mounted on a CryoLoop without cryoprotectant, diffraction patterns of the crystal are improved by using Paratone-N as cryoprotectant. Native X-ray diffraction data are collected to 2.4 A resolution using synchrotron radiation at station BL44XU of SPring-8. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = b = 217.48, c = 66.48 A. Assuming the presence of two molecules in the asymmetric unit, the VM value is 2.7 A3Da-1 and the solvent content is 54.1%. Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.30 56000
-
 SDS-PAGE Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.30 Thermococcus kodakarensis
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 hyperthermophilic archaeon
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Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.30 HiTrapQ HP column, Bio-Scale CHT20-I column, all purification procedures are carried out at 277 K. Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.30 GK glycerol kinase Thermococcus kodakarensis