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Literature summary extracted from

  • Faehnle, C.R.; Liu, X.; Pavlovsky, A.; Viola, R.E.
    The initial step in the archaeal aspartate biosynthetic pathway catalyzed by a monofunctional aspartokinase (2006), Acta Crystallogr. Sect. F, 62, 962-966.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 into pET-41a expression vector and expression in Escherichia coli Rosetta (DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.4 by hanging-drop vapor-diffusion method, in the presence of L-aspartate and MgADP, to 2.82–2.69 A resolution, N-terminal catalytic domain (residues 2-300) and a C-terminal regulatory domain (residues 310-470) joined through a hinge region (residues 301-309) Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-threonine
-
 Methanocaldococcus jannaschii
2.7.2.4 additional information L-lysine has no effect on activity at concentrations up to 10 mM, not inhibited by L-isoleucine and L-methionine Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 Mg2+ stabilizes the negative charge density on the terminal phosphoryl group and lowers the barrier towards transfer to the beta-carboxyl group of L-aspartate Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 58000
-
 dynamic light-scattering, addition up to 4000 mM guanidine-HCl leads to dissociation of the AK dimers into monomers Methanocaldococcus jannaschii
2.7.2.4 83000
-
 dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM causes a decrease in the AK particle size, dissociation into dimers Methanocaldococcus jannaschii
2.7.2.4 154000
-
 dynamic light-scattering, crystallization Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Methanocaldococcus jannaschii Q57991
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 by anion-exchange chromatography and gel filtration, more than 99% pure Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Methanocaldococcus jannaschii ADP + 4-phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 dimer dynamic light-scattering, addition of increasing levels of guanidine-HCl up to 2000 mM Methanocaldococcus jannaschii
2.7.2.4 monomer dynamic light-scattering, addition up to 4000 mM guanidine-HCl Methanocaldococcus jannaschii
2.7.2.4 tetramer crystallography, dynamic light-scattering, organized into a dimer of dimers, dimer interface mediated through both ACT subdomains, formation of the tetramer stabilized by the interaction of complementary residues from alpha4 of the catalytic domain Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 AK
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.2.4 37 70
-
 Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.4 12
-
 L-aspartate at 37°C Methanocaldococcus jannaschii
2.7.2.4 245
-
 L-aspartate at 70°C Methanocaldococcus jannaschii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.2.4 0.3
-
-
 Methanocaldococcus jannaschii L-threonine