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Literature summary extracted from

  • Li, J.; Xu, L.; Yang, F.
    Expression and characterization of recombinant thermostable alkaline phosphatase from a novel thermophilic bacterium Thermus thermophilus XM (2007), Acta Biochim. Biophys. Sin., 39, 844-850.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 expressed as a His6-tagged fusion protein in Escherichia coli Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 Ca2+ 2 mM, 16% inhibition Thermus thermophilus
3.1.3.1 Co2+ 2 mM, 25% inhibition Thermus thermophilus
3.1.3.1 Cu2+ 2 mM, 29% inhibition Thermus thermophilus
3.1.3.1 Fe2+ 2 mM, 89% inhibition Thermus thermophilus
3.1.3.1 Mn2+ 2 mM, 32% inhibition Thermus thermophilus
3.1.3.1 Zn2+ 2 mM, 51% inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.034
-
 4-nitrophenyl phosphate 75°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Co2+ 0.1 mM, 2.4fold increase in activity Thermus thermophilus
3.1.3.1 Fe2+ 0.1 mM, 1.25fold increase in activity Thermus thermophilus
3.1.3.1 Mg2+ 0.1 mM, 1.3fold increase in activity. 2 mM, 1.6fold increase in activity Thermus thermophilus
3.1.3.1 Mn2+ 0.1 mM, 1.3fold increase in activity Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 54700
-
 x * 54700, calculated from sequence Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Thermus thermophilus Q153J0
-
-
3.1.3.1 Thermus thermophilus XM Q153J0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Thermus thermophilus 4-nitrophenol + phosphate
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Thermus thermophilus XM 4-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 54700, calculated from sequence Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 75 80
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 45 90 45°C: about 50% of maximal activity, 90°C: about 70% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 50
-
 270 min, about 10% loss of activity Thermus thermophilus
3.1.3.1 80
-
 retains more than 50% activity after incubating for 6 h at 80ºC Thermus thermophilus
3.1.3.1 95
-
 60 min, about 45% loss of activity Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 12
-
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 11 12.5 11.0: about 50% of maximal activity, pH 12.5: about 65% of maximal activity Thermus thermophilus