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Literature summary extracted from
- beta-Glucosidase catalyzing specific hydrolysis of an iridoid beta-glucoside from Plumeria obtusa (2006), Acta Biochim. Biophys. Sin., 38, 563-570.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | 4-chloromercuribenzoate | 9% activation at 1 mM | Plumeria obtusa |  |
| 3.2.1.21 | Ca2+ | 15% activation at 1 mM | Plumeria obtusa | |
| 3.2.1.21 | EDTA | 10% activation at 1 mM | Plumeria obtusa | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Cu2+ | 21% inhibition at 1 mM | Plumeria obtusa | |
| 3.2.1.21 | D-glucono-1,5-lactone | 44% inhibition at 1 mM, 91% at 10 mM | Plumeria obtusa | |
| 3.2.1.21 | Fe3+ | slight inhibition of 8% at 1 mM | Plumeria obtusa | |
| 3.2.1.21 | Hg2+ | 14% inhibition at 1 mM | Plumeria obtusa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 1.02 | - |
plumieride coumarate beta-D-glucoside | pH 5.5, 37°C | Plumeria obtusa | |
| 3.2.1.21 | 5.04 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.5, 37°C | Plumeria obtusa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 54000 | - |
gel filtration | Plumeria obtusa |
| 3.2.1.21 | 60600 | - |
1 * 60600, SDS-PAGE | Plumeria obtusa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | plumieride coumarate beta-D-glucoside + H2O | Plumeria obtusa | an iridoid beta-glucoside from Plumeria obtusa, i.e. white frangipani, flower, the Plumeria obtusa beta-glucosidase shows high specificity for its natural substrate, plumieride coumarate glucoside | 13-O-coumarylplumieride + beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Plumeria obtusa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Plumeria obtusa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | native enzyme 217fold from flower to homogeneity by anion exchange and hydrophobic interaction chromatography, gel filtration, concanavalin A affinity chromatography and again anion exchange chromatography | Plumeria obtusa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | flower | - |
Plumeria obtusa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 7.38 | - |
purified enzyme | Plumeria obtusa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-fucoside + H2O | - |
Plumeria obtusa | 4-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Plumeria obtusa | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | additional information | substrate specificity, overview, poor hydrolysis of dalcochinin-8'-O-beta-glucoside, 4-methylumbelliferyl-beta-glucoside, and esculin, and no hydrolysis of alkyl-beta-glucosides, glucobioses, cyanogenic-beta-glucosides, steroid beta-glucosides, nor other iridoid beta-glucosides, including gonocaryoside A, or its derivative kingiside | Plumeria obtusa | ? | - |
? | |
| 3.2.1.21 | plumieride coumarate beta-D-glucoside + H2O | an iridoid beta-glucoside from Plumeria obtusa, i.e. white frangipani, flower, the Plumeria obtusa beta-glucosidase shows high specificity for its natural substrate, plumieride coumarate glucoside | Plumeria obtusa | 13-O-coumarylplumieride + beta-D-glucose | - |
? | |
| 3.2.1.21 | plumieride coumarate beta-D-glucoside + H2O | an iridoid beta-glucoside from Plumeria obtusa, i.e. white frangipani, flower, substrate and product identification and structure determination by NMR, overview | Plumeria obtusa | 13-O-coumarylplumieride + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | monomer | 1 * 60600, SDS-PAGE | Plumeria obtusa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | iridoid beta-glucoside | - |
Plumeria obtusa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 37 | - |
assay at | Plumeria obtusa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 13.9 | - |
plumieride coumarate beta-D-glucoside | pH 5.5, 37°C | Plumeria obtusa | |
| 3.2.1.21 | 30.7 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.5, 37°C | Plumeria obtusa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.5 | - |
- |
Plumeria obtusa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3 | 7.5 | 75% of maximal activity within the range of pH 4.5-6.5 | Plumeria obtusa |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Plumeria obtusa | isoelectric focusing | - |
4.9 |
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