Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sauerwald, A.; Zhu, W.; Major, T.A.; Roy, H.; Palioura, S.; Jahn, D.; Whitman, W.B.; Yates, J.R.; Ibba, M.; Soell, D.
    RNA-dependent cysteine biosynthesis in archea (2005), Science, 307, 1969-1972.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.73 gene Mj1678 or pscS, functional complementation of a cysteinyl-tRNACys synthase mutant strain, phylogenetic analysis, expression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.73 additional information the enzyme can synthesize Cys-tRNACys in a sepS deletion mutant when Na2S and O-phosphoserine are exogenously added, sepS encodes the O-phosphoseryl-tRNA synthetase, EC 6.1.1.B2 Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide Methanococcus maripaludis the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism containing a dispensable cysteinyl-tRNACys synthase, EC 6.1.1.16, overview L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide Methanocaldococcus jannaschii the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism lacking the cysteinyl-tRNACys synthase, EC 6.1.1.16, overview L-cysteinyl-tRNACys + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.73 Methanocaldococcus jannaschii
-
 gene Mj1678 or pscS
-
2.5.1.73 Methanococcus maripaludis A4FWT8 gene pcsS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.73 native enzyme to homogeneity Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism containing a dispensable cysteinyl-tRNACys synthase, EC 6.1.1.16, overview Methanococcus maripaludis L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide the natural sulfur donor is not characterized, the activity of SepCysS provides a means by which both cysteine and selenocysteine can be added to the genetic code, the enzyme is responsible for Cys-tRNACys synthesis together with the O-phosphoseryl-tRNA synthetase in the organism lacking the cysteinyl-tRNACys synthase, EC 6.1.1.16, overview Methanocaldococcus jannaschii L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide sulfide e.g. from Na2S, anaerobic reaction Methanocaldococcus jannaschii L-cysteinyl-tRNACys + phosphate
-
 ?
2.5.1.73 O-phospho-L-seryl-tRNACys + sulfide sulfide e.g. from Na2S, anaerobic reaction Methanococcus maripaludis L-cysteinyl-tRNACys + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.73 SepCysS
-
 Methanocaldococcus jannaschii
2.5.1.73 SepCysS
-
 Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.73 pyridoxal 5'-phosphate
-
 Methanocaldococcus jannaschii
2.5.1.73 pyridoxal 5'-phosphate
-
 Methanococcus maripaludis