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Literature summary extracted from

  • Feiden, S.; Stypa, H.; Wolfrum, U.; Wegener, G.; Kamp, G.
    A novel pyruvate kinase (PK-S) from boar spermatozoa is localized at the fibrous sheath and the acrosome (2007), Reproduction, 134, 81-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 additional information not activated by fructose 1,6-bisphosphate Sus scrofa
2.7.1.40 Triton X-100 50% increase of activity of the bound enzyme only Sus scrofa

General Stability

EC Number General Stability Organism
2.7.1.40 the enzyme cannot be solubilized in detergents, but trypsin without loss of activity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 additional information not inhibited by L-alanine Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.02 0.05 phosphoenolpyruvate bound enzyme Sus scrofa
2.7.1.40 0.02 0.03 phosphoenolpyruvate solubilized enzyme Sus scrofa
2.7.1.40 0.46
-
 ADP solubilized enzyme Sus scrofa
2.7.1.40 0.52 0.56 ADP bound enzyme Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 64500
-
 SDS-PAGE, subunit Sus scrofa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.1.40 additional information cannot be solubilized in detergents without loss of activity Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40 Fractogel EMD SO3- ion exchange chromatography and Fractogel EMD propyl (S) hydrophobic interaction chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.40 blood
-
 Sus scrofa
-
2.7.1.40 erythrocyte
-
 Sus scrofa
-
2.7.1.40 kidney
-
 Sus scrofa
-
2.7.1.40 spermatozoon in the acrosome of the sperm head and in the fibrous sheath of the principal piece of the flagellum Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.40 0.12
-
 sperm homogenate Sus scrofa
2.7.1.40 116.7
-
 after 972fold purification Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Sus scrofa ATP + pyruvate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.40 PK-S
-
 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.40 ADP
-
 Sus scrofa