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Literature summary extracted from

  • Fernandez, P.; Haouz, A.; Pereira, C.A.; Aguilar, C.; Alzari, P.M.
    The crystal structure of Trypanosoma cruzi arginine kinase (2007), Proteins Struct. Funct. Bioinform., 69, 209-212.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3 expressed in Escherichia coli BL21(DE3)pLysS cells Trypanosoma cruzi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.3 hanging drop method, structure of ligand-free TcAK is determined by molecular replacement methods and refined at 1.9 A resolution Trypanosoma cruzi
2.7.3.3 hanging drop vapour diffusion method using 2.5 M ammonium sulfate, and 0.1 M Tris-HCl, pH 7.5 Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Trypanosoma cruzi O96507
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3 Ni-column chromatography and Superdex-75 gel filtration Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.7.3.3 TcAK
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Trypanosoma cruzi